Development of homogeneous assay for simultaneous measurement of apoE-deficient, apoE-containing, and total HDL-cholesterol

Clinica Chimica Acta; International Journal of Clinical Chemistry
Yuji TakahashiHitoshi Chiba

Abstract

Pathophysiological role for high-density lipoprotein (HDL) subclasses remains to be elucidated. Homogeneous assay for simultaneous measurements of apoE-deficient HDL-cholesterol (HDL-C), apoE-containing HDL-C, and total HDL-C is desired, because apoE plays important roles in lipid metabolism. The proposed assay consists of a primary reaction to remove non-HDL-C, a secondary reaction to measure apoE-deficient HDL-C, and a tertiary reaction to measure apoE-containing HDL-C. The assay is completed within 10 min. For control study, 13% polyethylene glycol precipitation assay and phosphotungstate-dextran sulfate-magnesium precipitation assay were carried out. Good correlations between the control assays and the proposed assay was obtained in serum samples from patients without liver disease (n=33): r=0.987, 0.957, and 0.991 for apoE-deficient, apoE-containing, and total HDL-C, respectively. ApoE-containing HDL-C by the proposed method in healthy individuals (n=12) and patients with hyper-HDL-cholesterolemia (n=5) were 0.11±0.03 and 0.26±0.05 mmol/l (4.1±1.3 and 10.1±2.0 mg/dl), respectively. ApoE-containing HDL-C increased rapidly at >2.59 mmol/l (100 mg/dl) of total HDL-C, suggesting a unique regulating mechanism of apoE-containing...Continue Reading

References

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Nov 17, 2011·The New England Journal of Medicine·William E BodenWilliam Weintraub

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Citations

Aug 3, 2018·Annals of Clinical Biochemistry·Yuji TakahashiShu-Ping Hui
Jan 29, 2021·Arteriosclerosis, Thrombosis, and Vascular Biology·Minghao KouRon C Hoogeveen
Aug 18, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ryoko ShinohataShinichi Usui

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