Development of immunoassays for the detection of kanamycin in veterinary fields.

Journal of Veterinary Science
Yong JinMun-Han Lee

Abstract

Monoclonal antibody against kanamycin was prepared, and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk. The monoclonal antibody produced was identified to be IgG1, which has a kappa light chain. No cross-reactivity of the antibody was detected with other aminoglycosides, indicating that the monoclonal antibody was highly specific for kanamycin. Based on competitive direct ELISA, the detection limits of kanamycin were determined to be 1.1 ng/ml in PBS, 1.4 ng/ml in plasma, and 1.0 ng/ml in milk. The concentration of intramuscularly injected kanamycin was successfully monitored in rabbit plasma with competitive direct ELISA. Based on the colloidal gold-based immunochromatographic assay, the detection limits of kanamycin were estimated to be about 6-8 ng/ml in PBS, plasma, and milk. The immunochromatographic assay would be suitable for rapid and simple screening of kanamycin residues in veterinary medicine. Screened positives can be confirmed using a more sensitive laboratory method such as competitive direct ELISA. Therefore, the assays developed in this study could be used to complement each other as well as other laboratory findings. Moreo...Continue Reading

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Citations

Jun 27, 2007·BMC Microbiology·Cyrille J BonhommeDidier Raoult
Aug 15, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Xuewu LiHuanchun Chen
Dec 19, 2013·Journal of the American Veterinary Medical Association·Alexandra Moesta
Mar 8, 2020·Journal of the Science of Food and Agriculture·Mustafa Oguzhan Caglayan
Mar 6, 2012·Bioanalytical Reviews·Beate StrehlitzRegina Stoltenburg
Jul 7, 2021·PLoS Pathogens·Emily RomeisLorenzo Giacani
Sep 11, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Jiaojiao ZhouJuewen Liu
Apr 9, 2008·Journal of Agricultural and Food Chemistry·Yiqiang ChenXilong Xiao

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA

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Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.