Development of In Vitro Methodologies to Investigate Binding by Sodium Hyaluronate in Eye Drops to Corneal Surfaces

The Open Ophthalmology Journal
Udo BockReichl S

Abstract

To develop in vitro methods to assess binding by sodium hyaluronate in eye drops to corneal surfaces. Two different, complementary corneal binding set-ups were developed. In a dynamic in vitro model, confluent corneal epithelial cells (HCE-T) were assembled in chamber slides and a declining channel. A static model was constructed with ex vivo porcine corneas clamped in Franz cells. To test the predictive capacity of models, four different eye drops containing sodium hyaluronate were spiked with tritium-labeled sodium hyaluronate to standardize quantification. In both settings, eye drops were applied for 5 min and physiological conditions were mimicked by flushing with artificial tear fluid. Spreading experiments on HCE-T next to synthetic membranes were used for further characterization. Binding was more pronounced in dynamic HCE-T model. Three of the four eye drops demonstrated sigmoidal elution of sodium hyaluronate, suggesting pronounced binding. One solution eluted distinctly faster, likewise the buffer control. The static method produced a similar ranking but at lower levels. When eye drops in which phosphate buffer was replaced by citrate buffer (i.e., to prevent calcification) were used, binding was not influenced. All e...Continue Reading

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Citations

Mar 7, 2021·Carbohydrate Polymers·Gloria Huerta Ángeles, Kristina Nešporová

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Methods Mentioned

BETA
rheology
eye drop
confocal microscopy

Software Mentioned

TFOS
EVEIT
HYLO
COMOD

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