Development of Isocratic RP-HPLC Method for Separation and Quantification of L-Citrulline and L-Arginine in Watermelons

International Journal of Analytical Chemistry
Rasdin RidwanWan Mazlina Md Saad

Abstract

Watermelons (Citrullus lanatus) are known to have sufficient amino acid content. In this study, watermelons grown and consumed in Malaysia were investigated for their amino acid content, L-citrulline and L-arginine, by the isocratic RP-HPLC method. Flesh and rind watermelons were juiced, and freeze-dried samples were used for separation and quantification of L-citrulline and L-arginine. Three different mobile phases, 0.7% H3P04, 0.1% H3P04, and 0.7% H3P04 : ACN (90 : 10), were tested on two different columns using Zorbax Eclipse XDB-C18 and Gemini C18 with a flow rate of 0.5 mL/min and a detection wavelength at 195 nm. Efficient separation with reproducible resolution of L-citrulline and L-arginine was achieved using 0.1% H3P04 on the Gemini C18 column. The method was validated and good linearity of L-citrulline and L-arginine was obtained with R2 = 0.9956, y = 0.1664x + 2.4142 and R2 = 0.9912, y = 0.4100x + 3.4850, respectively. L-citrulline content showed the highest concentration in red watermelon of flesh and rind juice extract (43.81 mg/g and 45.02 mg/g), whereas L-arginine concentration was lower than L-citrulline, ranging from 3.39 to 11.14 mg/g. The isocratic RP-HPLC method with 0.1% H3P04 on the Gemini C18 column prove...Continue Reading

References

Jul 13, 2005·Journal of Chromatography. a·Agnes M Rimando, Penelope M Perkins-Veazie
Aug 6, 2005·Amino Acids·E CurisL Cynober
Jan 12, 2007·Cardiovascular Drug Reviews·Maritza J RomeroR William Caldwell
Mar 14, 2007·Nutrition·Julie K CollinsBeverly A Clevidence
Apr 22, 2008·Methods in Enzymology·Guoyao Wu, Cynthia J Meininger
Feb 2, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Soyoung ShinHo-Leung Fung
Sep 1, 2011·Analytical and Bioanalytical Chemistry·Bogusław Buszewski, Sylwia Noga
Apr 6, 2012·Journal of Pharmaceutical and Biomedical Analysis·Szabolcs FeketeDavy Guillarme
Aug 21, 2012·The Journal of Nutritional Biochemistry·Aruna PoduriAlan Daugherty
Apr 15, 2014·Journal of Neurosurgery. Pediatrics·Jung Won ChoiSeung-Ki Kim
May 31, 2014·BioMed Research International·Mohd Khairul Amran MohammadWan Mazlina Md Saad
Sep 23, 2014·Journal of Chromatographic Science·Endler M Borges

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Citations

Dec 31, 2020·BioMed Research International·Shahana WahidRafeeq Alam Khan
Sep 25, 2020·International Journal of Biological Macromolecules·Suet-Ying TamYun-Chung Leung

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

Chromeleon data
Chromeleon

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