PMID: 7540554Apr 1, 1995Paper

Development of kainic acid and N-methyl-D-aspartic acid toxicity in organotypic hippocampal cultures

Experimental Neurology
A J BruceM Baudry

Abstract

The excitotoxic effects of N-methyl-D-aspartic acid (NMDA) and kainic acid (KA) were studied in organotypic hippocampal slices maintained in vitro for various periods of time. Cultures aged to equivalent Postnatal Day (EPD) 10-12, 15-17, and 23-26 were exposed to 50 microM KA or 50 microM NMDA and were analyzed at 0, 3, 6, 9, 12, 24, 48 h, or 5 days after the initiation of the excitotoxin exposure. Neuronal injury was determined by: (1) propidium iodide (PI) uptake; (2) lactate dehydrogenase (LDH) release; (3) morphological damage in hematoxylin and eosin (H/E) stained sections; (4) loss of Nissl stain. Changes in PI uptake and LDH release after KA or NMDA treatment indicated that there was a developmental shift towards increasing sensitivity to KA toxicity during in vitro development, whereas cultures of all ages were equally sensitive to NMDA toxicity. The profile of damage in H/E-stained sections after treatment with KA or NMDA indicated a transient phase of damaged morphology at 12 and 24 h that was not evident after 5 days. To determine whether the disappearance of morphological manifestations of neuronal damage 5 days after treatment was due to recovery of morphology or to neuronal death, neuronal loss in Nissl-stained se...Continue Reading

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Citations

Sep 24, 1999·Journal of Neuroscience Research·A J Bruce-Keller
Oct 23, 2002·Journal of Neuroscience Research·Bruce Teter, J Wesson Ashford
Apr 15, 2004·Neurochemistry International·I E HolopainenT-K Kukko-Lukjanov
Dec 13, 2000·Brain Research. Developmental Brain Research·J D MarksX M Zhang
Nov 25, 1997·Brain Research. Developmental Brain Research·Y NakagamiN Matsuki
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·A J BruceM Baudry
May 1, 1997·The European Journal of Neuroscience·C BehlF Holsboer
Oct 15, 1998·Archives of Disease in Childhood·R C ScottB G Neville
May 3, 2012·Radiology Research and Practice·Ximena Wortsman
Aug 16, 2006·Neuro-degenerative Diseases·Li Zhou, Carol A Miller
Nov 26, 2008·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Helena Cimarosti, Jeremy M Henley
Apr 3, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Reymundo DominguezMichel Baudry
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M P LambertW L Klein
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y RongM Baudry
Feb 12, 2000·Neuropharmacology·M P Thomas, R A Morrisett
Oct 15, 2010·The International Journal of Neuroscience·Jinny J YoonLouise F B Nicholson
Jul 21, 2010·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Fahim EbrahimiFaramarz Dehghani
Jun 26, 2009·Progress in Neurobiology·Laura LossiAdalberto Merighi
Aug 1, 2015·Molecular Neurobiology·Qian LiJian Wang

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