Development of liposomal amphotericin B dry powder inhaler formulation

Drug Delivery
S P Shah, Ambikanandan Misra

Abstract

The purpose of our study was to prepare and optimize liposomal Amphotericin B (AMB) dry powder inhaler (DPI) formulation for treatment of invasive lung fungal infection. Liposomes were prepared by reverse phase evaporation technique using ethyl acetate and ethanol (1:1) as organic solvents to avoid a possible risk for human health and to impart adequate stability of the vesicles. Drug lipid ratio was 1:10 with membrane composition of hydrogenated soyaphosphatidylcholine; cholesterol and either saturated soyaphosphatidylglycerol (7:3:0.5) or stearylamine (1:1:0.1) was used to prepare negatively (AMB1) and positively (AMB2) charged liposomes, respectively. Liposomes were extruded through 2 microm polycarbonate membrane, separated from unentrapped drug and subjected to lyophilization using Tris buffer containing cryoprotectants in various mass ratios. Sucrose was found to be the best cryoprotectant for liposomal AMB in a mass ratio of lipid: sucrose at 1:5 for AMB1 and AMB2, respectively. Sorbolac 400 and sieved Pharmatose 325 M (500#) in varying mass ratios were used as carriers to prepare the liposomal DPI formulations and subjected to determination of angle of repose, compressibility index, dispersiblity index, water content, s...Continue Reading

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Citations

May 1, 2008·AAPS PharmSciTech·Mahavir ChouguleAmbikanandan Misra
Jan 8, 2010·International Journal of Nanomedicine·Heidi M MansourXiao Wu
Oct 15, 2013·Therapeutic Delivery·Ju DuHugh D C Smyth
Feb 26, 2009·Expert Opinion on Drug Delivery·T E Corcoran
Nov 7, 2014·Expert Opinion on Drug Delivery·Anne H de Boer, Paul Hagedoorn
Mar 6, 2008·Expert Opinion on Drug Delivery·Yuanyuan XieTimothy S Wiedmann
Feb 25, 2009·Expert Opinion on Drug Delivery·Ambikanandan MisraMahavir Chougule
Mar 5, 2011·Journal of Pharmaceutical Sciences·Rabkwan ChuealeeTeerapol Srichana
Dec 3, 2014·Advanced Drug Delivery Reviews·Qi Tony ZhouHak-Kim Chan
May 28, 2011·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Rabkwan ChuealeeTeerapol Srichana
Sep 13, 2005·International Journal of Antimicrobial Agents·Zahoor AhmadG K Khuller
Feb 14, 2014·Colloids and Surfaces. B, Biointerfaces·Dhruv ButaniAmbikanandan Misra
May 27, 2009·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Laura Kuiper, Elisabeth J Ruijgrok
Mar 2, 2010·Biological & Pharmaceutical Bulletin·Wen-Hua HuangLiang Liu
Jan 2, 2021·Frontiers in Bioengineering and Biotechnology·K M Aguilar-PérezHafiz M N Iqbal

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