PMID: 11254192Mar 20, 2001Paper

Development of liquid chromatographic method for the analysis of kanamycin residues in varicella vaccine using phenylisocyanate as a derivatization reagent

Journal of Chromatography. B, Biomedical Sciences and Applications
B H KimJ H Ok

Abstract

A liquid chromatographic method for the determination of the aminoglycoside kanamycin in varicella vaccine is described. Kanamycin sulfate was derived with phenylisocyanate (PIC) and triethylamine for 10 min at 70 degrees C and chromatographed on a alkylamide-bonded column, Suplex pKb-100. A derivative of kanamycin sulfate was attached to four phenylisocynato groups and that molecular mass was confirmed with liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS). The kanamycin-PIC derivative was found to have a retention time of 11.7 min using an eluent composed of 40% acetonitrile in water at 1.2 ml/min column flow-rate. Detection was at a wavelength of 240 nm. Recoveries ranging from 97.5 to 99.8% were found. The correlation coefficient was greater than 0.9998 over the range between 10 and 100 microg/ml. The method precision of within-day assay showed a 0.5 to 4.0% coefficient of variation (n = 5) ranging from 10 to 70 microg/ml of kanamycin concentration levels. Kanamycin-PIC derivative in reaction solution was stable for 24 h at room temperature. A simple and efficient method for the analysis of the kanamycin in varicella vaccine was developed and validated.

References

Citations

Nov 7, 2019·Magnetic Resonance in Chemistry : MRC·Daniel HudsonSimon F R Hinkley
Aug 6, 2020·Pharmaceutics·Bishal Raj AdhikariShyamal C Das

Related Concepts

phenyl isocyanate, 14C-labeled cpd
Bacteriocidal Agents
High Pressure Liquid Chromatography Procedure
Drug Residues
Indicators
Kantrex
Statistical Sensitivity
Mass Spectrometry
Reproducibility of Results
Isocyanates

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