Development of liquid chromatography-tandem mass spectrometry methods for the quantitation of Anisakis simplex proteins in fish

Journal of Chromatography. a
Christiane Kruse FæsteUwe Christians

Abstract

The parasite Anisakis simplex is present in many marine fish species that are directly used as food or in processed products. The anisakid larvae infect mostly the gut and inner organs of fish but have also been shown to penetrate into the fillet. Thus, human health can be at risk, either by contracting anisakiasis through the consumption of raw or under-cooked fish, or by sensitisation to anisakid proteins in processed food. A number of different methods for the detection of A. simplex in fish and products thereof have been developed, including visual techniques and PCR for larvae tracing, and immunological assays for the determination of proteins. The recent identification of a number of anisakid proteins by mass spectrometry-based proteomics has laid the groundwork for the development of two quantitative liquid chromatography-tandem mass spectrometry methods for the detection of A. simplex in fish that are described in the present study. Both, the label-free semi-quantitative nLC-nESI-Orbitrap-MS/MS (MS1) and the heavy peptide-applying absolute-quantitative (AQUA) LC-TripleQ-MS/MS (MS2) use unique reporter peptides derived from anisakid hemoglobin and SXP/RAL-2 protein as analytes. Standard curves in buffer and in salmon mat...Continue Reading

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Citations

Dec 27, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hongmin JiaPingli He
Aug 10, 2020·Journal of the Science of Food and Agriculture·Carla Godínez-GonzálezRoser Fisa
Jun 30, 2019·Developmental and Comparative Immunology·Wenche JohansenKnut Olav Strætkvern
Feb 24, 2021·International Journal of Food Microbiology·Regina KlapperUte Ostermeyer
Mar 27, 2018·Biochemical and Biophysical Research Communications·Qiong FanLine M Grønning-Wang

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