Abstract
Diagnosis of canine babesiosis, caused by Babesia gibsoni is difficult, especially in chronically infected dogs. A loop mediated isothermal amplification (LAMP) assay was developed and standardized by using four oligonucleotide primers targeting the hypervariable region of 18S rRNA gene (GenBank Acc. no. KC461261). The primers specifically amplified B. gibsoni DNA, while no amplification was detected with DNA from non-infected dogs as well as from dogs infected with Babesia canis vogeli, Hepatozoon canis, Ehrlichia canis and Trypanosoma evansi. The assay could detect 1.35 × 10(-7) parasitaemia and 10(-4) dilution of recombinant plasmid, equivalent to 12 pg of target DNA. All the samples were tested by nested PCR as well as LAMP assay. LAMP was found to be 10 times more sensitive than nested PCR targeting the same gene. Out of 75 suspected field samples, collected from different parts of the country, LAMP could detect B. gibsoni in 43 samples, while nested PCR and microscopy could detect 37 and 23 samples, respectively. High sensitivity, specificity and rapidity of LAMP assay may be exploited for screening large number of samples in a field setting.
References
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Feb 24, 2001·The Journal of Veterinary Medical Science·H AnoR Harasawa
Nov 16, 2001·Biochemical and Biophysical Research Communications·Y MoriT Notomi
Sep 6, 2003·Journal of Immunological Methods·Joseph A WhelanMichael A Whelan
Sep 6, 2003·Journal of Clinical Microbiology·Adam J BirkenheuerEdward B Breitschwerdt
Jun 9, 2004·Journal of Clinical Microbiology·Hiromi IkadaiTakashi Oyamada
May 24, 2005·Veterinary Parasitology·Oriel M M ThekisoeChihiro Sugimoto
Sep 16, 2006·Veterinary Parasitology·Andy AlhassanIkuo Igarashi
Jul 10, 2007·Applied and Environmental Microbiology·Panagiotis KaranisNoborou Inoue
Aug 21, 2007·Veterinary Parasitology·Gabriel Oluga AbogeXuenan Xuan
Dec 25, 2007·Experimental Parasitology·Youn-Kyoung GooXuenan Xuan
Aug 22, 2008·Reviews in Medical Virology·Manmohan ParidaKouichi Morita
Apr 10, 2009·Veterinary Parasitology·D KrastevaJ Sri Widada
Aug 6, 2009·International Journal for Parasitology·Oriel M M ThekisoeNoboru Inoue
Sep 8, 2009·Trends in Parasitology·Panagiotis Karanis, Jerry Ongerth
Mar 26, 2010·Veterinary Parasitology·L X WangJ L Zhao
Oct 12, 2010·The Veterinary Clinics of North America. Small Animal Practice·Peter J Irwin
Jul 17, 2012·Veterinary Parasitology·Youn-Kyoung GooXuenan Xuan
Dec 26, 2012·Veterinary Parasitology·Jorge OyhenartJavier D Breccia
Aug 15, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·M MandalG V P P S Ravi Kumar
Sep 23, 2014·Veterinary Parasitology·Mrityunjay MandalOpinder K Raina
Citations
Feb 28, 2016·Veterinary Parasitology·Yimin YangAifang Du
Dec 1, 2015·Ticks and Tick-borne Diseases·Mingming LiuXuenan Xuan
Sep 10, 2019·3 Biotech·Gaurav VermaSwarup Kumar Chakrabarti
Dec 13, 2019·Environmental Science and Pollution Research International·Liang WangRui Sun
Nov 16, 2019·Acta Parasitologica·Manraj Deep SinghShitanshu Shekar Rath
Dec 1, 2019·BMC Veterinary Research·I-Li LiuHui-Wen Chen
Feb 14, 2018·Journal of Clinical Microbiology·Noah HullBrant Schumaker
Mar 3, 2021·Parasitology Research·Archana UpadhyayQian Han
Mar 14, 2021·Parasitology Research·Binod KumarVijay L Parmar