Development of loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Penicillium nordicum in dry-cured meat products

International Journal of Food Microbiology
M FerraraA Susca

Abstract

The need of powerful diagnostic tools for rapid, simple, and cost-effective detection of food-borne fungi has become very important in the area of food safety. Currently, several isothermal nucleic acid amplification methods have been developed as an alternative to PCR-based analyses. Loop-mediated isothermal amplification (LAMP) is one of these innovative methods; it requires neither gel electrophoresis to separate and visualize the products nor expensive laboratory equipment and it has been applied already for detection of pathogenic organisms. In the current study, we developed a LAMP assay for the specific detection of Penicillium nordicum, the major causative agent of ochratoxin A contamination in protein-rich food, especially dry-cured meat products. The assay was based on targeting otapksPN gene, a key gene in the biosynthesis of ochratoxin A (OTA) in P. nordicum. Amplification of DNA during the reaction was detected directly in-tube by color transition of hydroxynaphthol blue from violet to sky blue, visible to the naked eye, avoiding further post amplification analyses. Only DNAs isolated from several P. nordicum strains led to positive results and no amplification was observed from non-target OTA and non OTA-producing...Continue Reading

References

Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
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Jun 14, 2008·Journal of Clinical Microbiology·Joshua HillAbhay Vats
Jul 25, 2008·Toxicon : Official Journal of the International Society on Toxinology·Maricel Marin-KuanBenoît Schilter
Sep 16, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·S D StoevP A Steenkamp
May 6, 2010·International Journal of Food Microbiology·Ludwig Niessen, Rudi F Vogel
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Jun 1, 2006·Mycotoxin Research·R GeisenA Karolewiez
Oct 5, 2014·Fungal Genetics and Biology : FG & B·Antonia SuscaAntonio Moretti

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Citations

Dec 1, 2015·International Journal of Food Microbiology·Vincenzo LippolisGiancarlo Perrone
Sep 9, 2019·Mycotoxin Research·Paula RodriguesAlfredo Teixeira
Nov 23, 2016·Analytical and Bioanalytical Chemistry·Xiaofu WangJunfeng Xu
Nov 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Yogesh KumarPranita Jaiswal

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