Development of magnetic bead based sample extraction coupled polymerase spiral reaction for rapid on-site detection of Chikungunya virus.

Scientific Reports
Shashi SharmaDevendra Kumar Dubey

Abstract

The molecular detection system has evolved over last two decades and is rapidly replacing the conventional confirmatory techniques in diagnostic virology. However the major limitation in implementation of available molecular detection assays is the non availability of field deployable nucleic acid isolation platform coupled with gene amplification technique. The rapid and early molecular detection is crucial for employing effective measure against many viral infections. The re-emergence of chikungunya virus (CHIKV) has led to epidemics since 2004 in several parts of the world including India. The main association of CHIKV with severe arthritis and long-lasting arthralgia and closely mimics symptoms of Dengue and Zika virus infection requiring laboratory confirmation. In this study, a simple magnetic bead based ribonucleic acid extraction method was optimized, which was coupled with isothermal polymerase spiral reaction (PSR) technique for early and rapid detection. Subsequently, the polymerase spiral reaction reagents were converted to dry down format that led to a rapid user friendly field compatible sample processing to answer method for rapid and onsite detection of Chikungunya virus. Both the methods were evaluated with a p...Continue Reading

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Citations

Jun 26, 2020·The Cochrane Database of Systematic Reviews·Jonathan J DeeksUNKNOWN Cochrane COVID-19 Diagnostic Test Accuracy Group
Jan 22, 2021·Expert Review of Molecular Diagnostics·Taylor J MoehlingRobert J Meagher
Jul 14, 2021·Chemical Reviews·Min LiQiang Xia

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Datasets Mentioned

BETA
AY112987
J02363

Methods Mentioned

BETA
nucleic acid amplification
PCR
nucleic acid extraction
electrophoresis
ELISA
nucleic acid purification
reverse transcription-PCR
in vitro transcription

Software Mentioned

LoopAmp
ASSURED
Lasergene
ClustalW

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