Development of mammalian serum albumin affinity purification media by peptide phage display

Biotechnology Progress
Aaron K SatoRobert Ladner

Abstract

Several phage isolates that bind specifically to human serum albumin (HSA) were isolated from disulfide-constrained cyclic peptide phage-display libraries. The majority of corresponding synthetic peptides bind with micromolar affinity to HSA in low salt at pH 6.2, as determined by fluorescence anisotropy. One of the highest affinity peptides, DX-236, also bound well to several mammalian serum albumins (SA). Immobilized DX-236 quantitatively captures HSA from human serum; mild conditions (100 mM Tris, pH 9.1) allow release of HSA. The DX-236 affinity column bound HSA from human serum with a greater specificity than does Cibacron Blue agarose beads. In addition to its likely utility in HSA and other mammalian SA purifications, this peptide media may be useful in the proteomics and medical research markets for selective removal of mammalian albumin from serum prior to mass spectrometric and other analyses.

Citations

Sep 24, 2004·Journal of Molecular Recognition : JMR·Tony J FlemingDaniel J Sexton
Dec 2, 2010·Journal of Materials Science. Materials in Medicine·Sanjukta Guha Thakurta, Anuradha Subramanian
May 15, 2013·Colloids and Surfaces. B, Biointerfaces·Muge AndacAdil Denizli
Feb 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·Adam R MezoAlan J Bitonti
Oct 20, 2005·Journal of Bioscience and Bioengineering·Fumiaki UchiyamaNoritaka Tokui
Jan 1, 2009·Human Genomics and Proteomics : HGP·Kondethimmanahalli Chandramouli, Pei-Yuan Qian
Nov 27, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ting LeiJen-Fu Chiu
Mar 3, 2006·Analytical Biochemistry·Dina WassafDaniel J Sexton
Jul 15, 2015·Analytical and Bioanalytical Chemistry·Maxime Boulet-AudetSergei G Kazarian
Jul 11, 2012·International Journal of Proteomics·Lisandra E de Castro BrásMerry L Lindsey
Jan 18, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dmitri SitnikovJoanna M Hunter
Jul 6, 2004·Journal of Chromatography. a·Brian D KelleyArthur Ley
Aug 15, 2003·Molecular & Cellular Proteomics : MCP·Radhakrishna S TirumalaiTimothy D Veenstra
Feb 3, 2004·Journal of Receptor and Signal Transduction Research·Michael Szardenings
Mar 11, 2005·Proteomics·Thierry BaussantKeith Rose
May 5, 2012·Journal of Separation Science·Muge AndacAdil Denizli
Jan 21, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Miha VodnikMojca Lunder
Dec 29, 2006·Contrast Media & Molecular Imaging·Michael F Tweedle
Apr 4, 2007·Mass Spectrometry Reviews·Xuewu ZhangFeng Chen
May 29, 2015·Journal of Animal Science·J K GrubbsS M Lonergan
Aug 10, 2005·Protein Engineering, Design & Selection : PEDS·A ShrivastavaA D Nunn
Jun 6, 2009·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Roland E Kontermann
Dec 30, 2017·Expert Review of Proteomics·Merry L LindseyKristine Y DeLeon-Pennell
Feb 9, 2021·ACS Omega·Yuhao MaLarry D Unsworth
Jun 26, 2009·Accounts of Chemical Research·Michael F Tweedle
Dec 22, 2009·Journal of Proteome Research·Ravi C DwivediJohn A Wilkins
Aug 8, 2021·International Journal of Molecular Sciences·Katie DunphyDespina Bazou
Nov 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anna Bodzon-KulakowskaJerzy Silberring

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