Development of metronidazole-resistant lines of Blastocystis sp.

Parasitology Research
Linda DunnJacqueline A Upcroft

Abstract

Metronidazole (MTR) is frequently used for the treatment of Blastocystis infections, but with variable effectiveness, and often with treatment failures as a possible result of drug resistance. We have developed two Blastocystis MTR-resistant (MTR(R)) subtype 4 WR1 lines (WR1-M4 and WR1-M5), with variable susceptibility to a panel of anti-protozoal agents including various 5-nitroimidazoles, nitazoxanide and furazolidone. WR1-M4 and WR1-M5 were developed and assessed over an 18-month period and displayed persistent MTR resistance, being more than 2.5-fold less susceptible to MTR than the parent isolate. The MTR(R) lines grew with a similar g time to WR1, but were morphologically less consistent with a mixture of size. All Blastocystis isolates and the MTR(R) lines were most susceptible to the 5-nitroimidazole drug ronidazole. WR1-M5 was apparently cross-resistant to satranidazole and furazolidone, and WR1-M4 was cross-resistant to nitazoxanide. These MTR(R) lines now provide a valuable tool for the continued assessment of the efficacy and mechanism of action of new and established drugs against a range of Blastocystis sp. subtypes, in order to identify a universally effective drug and to facilitate understanding of the mechanism...Continue Reading

References

Apr 1, 1991·The Journal of Antimicrobial Chemotherapy·L A Dunn, P F Boreham
Feb 1, 1989·International Journal for Parasitology·L A DunnD J Stenzel
Nov 1, 1984·The Journal of Antimicrobial Chemotherapy·P F BorehamR W Shepherd
Apr 4, 1996·Nature·J D SilbermanC G Clark
Jan 1, 1997·Parasitology Research·X Q ChenE H Yap
Jun 5, 1999·Tropical Medicine & International Health : TM & IH·K HareshS Saminathan
Nov 24, 1999·The American Journal of Gastroenterology·U Z OkA A Kilimcioğlu
Jan 9, 2001·Clinical Microbiology Reviews·P Upcroft, J A Upcroft
May 17, 2001·Antimicrobial Agents and Chemotherapy·J A Upcroft, P Upcroft
Dec 26, 2001·Bioinformatics·S KumarM Nei
Mar 26, 2003·Journal of Travel Medicine·Luciano NigroBruno Cacopardo
Jul 26, 2003·The Journal of Parasitology·Andrea GiacomettiGiorgio Scalise
Aug 9, 2003·Revista Da Sociedade Brasileira De Medicina Tropical·Sérgio CimermanWilma Assunção Iuliano
Jul 15, 2004·British Journal of Biomedical Science·J YakoobM Asim Beg
May 26, 2005·Parasitology Research·Davood Dorostkar MoghaddamM Azami
Oct 20, 2005·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Jean-François RossignolAzza M Younis
Dec 27, 2005·Antimicrobial Agents and Chemotherapy·Jacqueline A UpcroftPatrice Vanelle
Aug 3, 2006·Nature Clinical Practice. Gastroenterology & Hepatology·Michael J G Farthing
Dec 2, 2006·International Journal of Antimicrobial Agents·Linda A DunnJacqueline A Upcroft
Jan 24, 2007·Trends in Parasitology·C Rune StensvoldC Graham Clark
Dec 8, 2007·Parasitology Research·Kenneth H S WongKevin S W Tan
Jul 2, 2008·European Journal of Medicinal Chemistry·Maxime D CrozetPatrice Vanelle
Oct 22, 2008·Parasites & Vectors·Kenneth F BooromMorris S Jones
Mar 18, 2009·Parasitology Research·Laetitia SouppartEric Viscogliosi
Oct 17, 2009·Journal of Clinical Gastroenterology·Christen R StensvoldRebecca J Traub
Jan 22, 2010·Veterinary Parasitology·Unaiza ParkarR C Andrew Thompson
Feb 24, 2010·Parasitology Research·Javed YakoobRustam Khan
Apr 7, 2010·Parasitology International·Christian VogelbergChristoph Pöhlmann
May 12, 2010·International Journal of Antimicrobial Agents·Linda A DunnJacqueline A Upcroft
Oct 6, 2010·Parasitology Research·Dina M Abdel HameedNehal Mohamed Zuel-Fakkar
Jan 18, 2011·Journal of Feline Medicine and Surgery·Dana N LeVineRebecca B Hayes
Feb 11, 2011·Current Infectious Disease Reports·Kevin S W TanPaul A Macary
Mar 29, 2011·Genome Biology·France DenoeudHicham El Alaoui
Aug 16, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thierry JuspinPatrice Vanelle

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Citations

Jun 3, 2014·Gut Pathogens·Tamalee RobertsJohn Ellis
Jan 19, 2016·Parasitology International·Özgür KurtMehmet Tanyüksel
Mar 19, 2015·Parasitology Research·Hend Aly El-Taweel
May 20, 2015·Antimicrobial Agents and Chemotherapy·Tamalee RobertsDamien Stark

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