Development of microarray and multiplex polymerase chain reaction assays for identification of serovars and virulence genes in Salmonella enterica of human or animal origin

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Greg PetersonSanjeev Narayanan

Abstract

Salmonella enterica is an important enteric pathogen consisting of many serovars that can cause severe clinical diseases in animals and humans. Rapid identification of Salmonella isolates is especially important for epidemiologic monitoring and controlling outbreaks of disease. Although immunologic and DNA-based serovar identification methods are available for rapid identification of isolates, they are time consuming or costly or both. In the current study, 2 molecular methods for identification of Salmonella serovars were developed and validated. A 70-mer oligonucleotide spotted microarray was developed that consisted of probes that detected genes responsible for genetic variation among isolates of Salmonella that can be used for serotyping. A multiplex polymerase chain reaction (PCR) assay was also developed, which is capable of identifying 42 serovars, thus providing a valuable prediction of the pathogenicity of the isolates by detecting the presence of virulence genes sseL, invA, and spvC. The gene spvC was the best predictor of pathogenicity. In a blind study, traditional serologic methods were correlated at 93.3% with the microarray-based method and 100% with the multiplex PCR-based serovar determination.

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Citations

Aug 23, 2011·Applied and Environmental Microbiology·Pierre WattiauSophie Bertrand
Sep 16, 2014·Diagnostic Microbiology and Infectious Disease·Catherine YoshidaRoderick M Card
Jan 18, 2015·Journal of Microbiological Methods·Joelle K SalazarWei Zhang
Dec 7, 2010·International Journal of Food Microbiology·Bin LiuXianming Shi
Nov 16, 2013·Critical Reviews in Microbiology·Chunlei ShiAndrea Isabel Moreno Switt
Dec 18, 2016·Environmental Science and Pollution Research International·Lincohn ZappeliniMaria Tereza Pepe Razzolini
Nov 7, 2017·Foodborne Pathogens and Disease·Karen Apellanis BorgesVladimir Pinheiro do Nascimento
Jan 1, 2014·BMC Microbiology·Flávio KrzyzanowskiMaria Tereza Pepe Razzolini
Mar 25, 2020·Microbial Biotechnology·Youming ShenHaifei Li

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Datasets Mentioned

BETA
AE008802

Methods Mentioned

BETA
PCR
nucleic acid hybridization
electrophoresis
chips
chip

Software Mentioned

OligoWiz
Primer 3
AutoDimer
GenePix Array List ( GAL
GenePix Report
BLAST
TIGR Multi - Experiment Viewer ( TMEV )

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