Development of microsatellite markers and analysis of intraspecific genetic variability in chickpea (Cicer arietinum L.)

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Niroj Kumar SethySabhyata Bhatia

Abstract

Paucity of polymorphic molecular markers in chickpea (Cicer arietinum L.) has been a major limitation in the improvement of this important legume. Hence, in an attempt to develop sequence-tagged microsatellite sites (STMS) markers from chickpea, a microsatellite enriched library from the C. arietinum cv. Pusa362 nuclear genome was constructed for the identification of (CA/GT)n and (CT/GA)n microsatellite motifs. A total of 92 new microsatellites were identified, of which 74 functional STMS primer pairs were developed. These markers were validated using 9 chickpea and one C. reticulatum accession. Of the STMS markers developed, 25 polymorphic markers were used to analyze the intraspecific genetic diversity within 36 geographically diverse chickpea accessions. The 25 primer pairs amplified single loci producing a minimum of 2 and maximum of 11 alleles. A total of 159 alleles were detected with an average of 6.4 alleles per locus. The observed and expected heterozygosity values averaged 0.32 (0.08-0.91) and 0.74 (0.23-0.89) respectively. The UPGMA based dendrogram was able to distinguish all the accessions except two accessions from Afghanistan establishing that microsatellites could successfully detect intraspecific genetic diver...Continue Reading

References

Jan 1, 1987·Acta Anatomica·M ArighiF D Horney
Feb 1, 1994·Nature Genetics·R I Richards, G R Sutherland
Jun 6, 1996·Nature·W MessierC B Stewart
Aug 1, 1996·Nature Genetics·W AmosD C Rubinsztein
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·L JinE Mignot
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·S KruglyakC F Aquadro
May 8, 1999·Genome Génome / Conseil National De Recherches Canada·B HüttelG Kahl
May 20, 1999·Molecular Biology and Evolution·J S TaylorF Breden
May 5, 2000·Journal of Molecular Evolution·Y ZhuJ E Strassmann
Nov 10, 2000·Chromosoma·C Schlötterer
Aug 29, 2001·Molecular Genetics and Genomics : MGG·S M Udupa, M Baum
Mar 21, 2002·Molecular Ecology·L ZaneT Patarnello
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M. IruelaT. Millán
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y. MatsuokaJ. Doebley
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·A. SudupakA. Kence
Mar 20, 2003·Molecular Biology and Evolution·Richard M SiblyJohn C Whittaker
May 17, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·H Flandez-GalvezP W J Taylor
May 21, 2003·Molecular Ecology·J SquirrellW Powell
May 21, 2003·Molecular Genetics and Genomics : MGG·T Pfaff, G Kahl
Nov 8, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S RiazC P Meredith
Jan 24, 2004·The Quarterly Review of Biology·Shahal AbboAvi Gopher
Apr 7, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M E FergusonS Mitchell
Apr 7, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·A J LoweK J Edwards
Feb 16, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J LichtenzveigH-B Zhang
Mar 1, 1992·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·F AhmadA E Slinkard
Jul 1, 1995·World Journal of Microbiology & Biotechnology·P Winter, G Kahl

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Citations

May 16, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P RadhikaV S Gupta
Oct 24, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Swarup K ParidaTrilochan Mohapatra
Nov 21, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Shalu ChoudharySabhyata Bhatia
Jan 26, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Spurthi N NayakRajeev K Varshney
Mar 9, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Neha GujariaRajeev K Varshney
Feb 4, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Shalu ChoudharyShefali Gupta
Aug 23, 2006·Molecular Genetics and Genomics : MGG·Valérie PoncetPerla Hamon
Apr 1, 2010·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Subhojit DattaShiv Kumar
Aug 7, 2012·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Rashmi GaurSabhyata Bhatia
May 2, 2013·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Alice KujurSwarup K Parida
May 3, 2011·Genome Génome / Conseil National De Recherches Canada·Tae-Hwan JunM A Rouf Mian
Dec 12, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Rajeev K VarshneyK P Viswanatha
Jun 24, 2014·BMC Bioinformatics·Dadakhalandar DoddamaniRajeev K Varshney
Aug 12, 2015·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Cong-Ying YuanXin-Hong Guo
Jan 1, 2008·Biotechnology & Genetic Engineering Reviews·Rakesh SinghN K Singh
Aug 12, 2015·Scientific Reports·Sabiha ParweenDebasis Chattopadhyay
Jun 8, 2012·Plant Biotechnology Journal·Shalu JhanwarMukesh Jain
Mar 3, 2017·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·C De GiovanniC Lotti
Oct 1, 2014·Functional Plant Biology : FPB·Mahendar ThudiRajeev K Varshney
Jul 22, 2017·Frontiers in Plant Science·Bingi P MallikarjunaPooran M Gaur

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