Development of Microsatellite Markers for a Dioecious Herpetospermum pedunculosum (Cucurbitaceae)

Evolutionary Bioinformatics Online
Zhu-Qing ChenYuan-Wen Duan

Abstract

Understanding the evolution of flower diversity is a central topic in plant evolutionary ecology, and natural selection on floral traits via male fitness could be estimated quantitatively using microsatellites. Here, based on RNA sequencing, we developed simple sequence repeat primers and verified polymorphisms in 2 wild populations of Herpetospermum pedunculosum (Cucurbitaceae), a dioecious annual plants native to the Himalaya Mountains. A total of 131 paired primers were designed; 15 paired primers were found to be polymorphic, with the expected heterozygosity varying between 0.280 and 0.767. We also identified 58 genotypes in 20 plants from the 2 populations. Conclusively, these primers could be effective in examining male fitness and population genetic structure of H pedunculosum in future studies.

References

Jul 2, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Spencer C H Barrett
Jul 18, 2006·Nucleic Acids Research·Jia YeJun Wang
May 29, 2007·Nucleic Acids Research·Yuki MoriyaMinoru Kanehisa
Oct 20, 2007·Trends in Biotechnology·Prakash C SharmaGünter Kahl
Apr 19, 2008·Evolution; International Journal of Organic Evolution·Kathryn A Hodgins, Spencer C H Barrett
May 17, 2008·International Journal of Plant Genomics·Ana Conesa, Stefan Götz
Jun 26, 2009·The New Phytologist·Lawrence D Harder, Steven D Johnson
Jan 6, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Spencer C H Barrett
Oct 14, 2010·The New Phytologist·Yuval Sapir, W Scott Armbruster
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Apr 24, 2012·Evolution; International Journal of Organic Evolution·Mason W Kulbaba, Anne C Worley
May 20, 2015·Evolutionary Bioinformatics Online·Kuangyu WangJeffrey L Thorne
Nov 26, 2015·Evolution; International Journal of Organic Evolution·Emily J Austen, Arthur E Weis
Apr 8, 2017·Genomics, Proteomics & Bioinformatics·Yanqing WangWenming Zhao
Oct 27, 2018·Nucleic Acids Research·UNKNOWN BIG Data Center Members
Apr 26, 2019·Methods in Molecular Biology·Mathieu SeppeyEvgeny M Zdobnov
Oct 6, 2019·Annals of Botany·Dorothy A ChristopherJeffrey D Karron

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Methods Mentioned

BETA
PCR
PCRs

Software Mentioned

Blast2GO
Primer
BUSCO
WEGO
BLASTx
KEGG
Trinity

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