Development of microwave assisted spectrophotometric method for the determination of glucose

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Asif AliAmir Zada

Abstract

A spectrophotometric method was developed based on the microwave assisted synthesis of Maillard product. Various conditions of the reaction were optimized by varying the relative concentration of the reagents, operating temperature and volume of solutions used in the reaction in the microwave synthesizer. The absorbance of the microwave synthesized Maillard product was measured in the range of 360-740nm using UV-Visible spectrophotometer. Based on the maximum absorbance, 370nm was selected as the optimum wave length for further studies. The LOD and LOQ of glucose was found 3.08μgmL(-1) and 9.33μgmL(-1) with standard deviation of ±0.05. The developed method was also applicable to urine sample.

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