Development of mini-gel technology in two-dimensional electrophoresis for mass-screening of samples: application to tears

Electrophoresis
M J GlassonK L Williams

Abstract

Despite the extensive literature available on tear proteins and lipids, very little has been reported on the tear fluid as a whole and it's changes in contact lens wear or ocular diseased patients. Initially a human reflex tear two-dimensional map was created by Molloy et al. (Electrophoresis 1997, 18, 2811-2815), using this information a process for mass-screening was established. The large format two-dimensional technique was evaluated, using a basal tear reference map, and modified to describe a fast, efficient and cost effective method of protein separation. The use of one pH 3-10 18 cm nonlinear immobilised pH gradient (IPG) strip and two mini-gels for the second-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) results in an effective separation of tear proteins which will be applied in diagnostic studies of tear samples.

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Citations

Jun 27, 2001·Electrophoresis·S HerberA J Augustin
Jun 3, 2005·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Johan BoursWinrich Breipohl
Sep 21, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·K Govindaraju, D K Lloyd
Dec 17, 2002·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·M J GlassonM DP Willcox
Jun 27, 2012·Progress in Retinal and Eye Research·Lei Zhou, Roger W Beuerman
Jan 1, 2008·Research in Veterinary Science·Carla de Freitas CamposMark D P Willcox
Aug 1, 2007·Proteomics. Clinical Applications·Franz H GrusNorbert Pfeiffer
Dec 2, 2005·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Franz H GrusNorbert Pfeiffer
Nov 2, 2011·Clinical Biochemistry·Hyun-Jung KimChan-Wha Kim
Oct 6, 2006·European Journal of Clinical Nutrition·U SenarathK E Agho

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