Development of molecular approach based on PCR assay for detection of histamine producing bacteria

Journal of Food Science and Technology
Karn WongsariyaMullika Traidej Chomnawang

Abstract

Histamine fish poisoning becomes highly concern not only in public health but also economic aspect. Histamine is produced from histidine in fish muscles by bacterial decarboxylase enzyme. Several techniques have been developed to determine the level of histamine in fish and their products but the effective method for detecting histamine producing bacteria is still required. This study was attempted to detect histamine producing bacteria by newly developed PCR condition. Histamine producing bacteria were isolated from scombroid fish and determined the ability to produce histamine of isolated bacteria by biochemical and TLC assays. PCR method was developed to target the histidine decarboxylase gene (hdc). The result showed that fifteen histamine producing bacterial isolates and three standard strains produced an amplicon at the expected size of 571 bp after amplified by PCR using Hdc_2F/2R primers. Fifteen isolates of histamine producing bacteria were classified as M. morganii, E. aerogenes, and A. baumannii. The lowest detection levels of M. morganii and E. aerogenes were 10(2) and 10(5) Cfu/mL in culture media and 10(3) and 10(6) Cfu/mL in fish homogenates, respectively. The limit of detection by this method was clearly shown t...Continue Reading

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Citations

Jul 29, 2016·Current Opinion in Allergy and Clinical Immunology·Erminia RidoloGiorgio Ricci
Aug 24, 2017·Journal of Forensic Sciences·Yang YuShijun Hong
Aug 23, 2021·Toxicon : Official Journal of the International Society on Toxinology·James M Hungerford

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