Development of molecular diagnostic protocols for detecting three types of Entamoeba from diarrheal and asymptomatic pigs and environmental moist soils

Parasitology Research
Yoshimasa HirashimaTomoyuki Shibahara

Abstract

Entamoeba suis and Entamoeba polecki subtypes (ST) 1 and 3 have recently been implicated in disease outbreaks in pigs. However, the distributions of these parasites in Japan and the potential sources of infection on farms still remain unclear. Here, we examined a farm of fattening/growing pigs with abnormal feces in Kagoshima Prefecture, Japan, and found the presence of parasites in the farm environment. Examination of intestinal tissues from pigs presenting with ulcerative colitis revealed a large number of trophozoites that had invaded the lesions. We identified single and mixed infections of E. suis and E. polecki ST1 and ST3 in paraffin sections or fecal samples from affected pigs. Two subtypes of Entamoeba were identified using four primer sets by PCR and sequencing. The parasites were detected in moist soil samples obtained around the drinking water source or puddles, implicating transmission of cysts via contaminated soils. Additionally, we found evidence of Entamoeba spp. and coinfections in surveyed pigs without any diarrhea at two neighboring farms. Our results establish methods for successfully identification of parasites, including cases in which multiple infections are present.

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Citations

Nov 30, 2019·BioMed Research International·Ting JiDong-Hui Zhou
Feb 27, 2020·The Journal of Veterinary Medical Science·Hirotaka ItoTomoyuki Shibahara
Mar 2, 2018·Journal of Clinical Microbiology·Christen Rune StensvoldMarianne Lebbad

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Datasets Mentioned

BETA
AJ566411
DQ286374
FR686456
DQ286372
FR686385
FR686384

Methods Mentioned

BETA
ELISA
PCR

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