Development of molecular genetic interventions for HIV infection

Current Protocols in Human Genetics
C WoffendinG J Nabel

Abstract

This unit includes a set of protocols for the ex vivo transfer of genes into CD4+ T cells, to be used in the initial evaluation of genes protecting against HIV infection in gene therapy protocols. The describes isolating and expanding CD4+ T cells from the patient. The cells are then transduced by either retroviral transduction or particle-mediated gene transfer and reinfused into the patient. To monitor the effectiveness of gene transfer, genomic DNA is prepared from the patient's cells. Detection of vector DNA by PCR analysis of the patient's genomic DNA following gene transfer is also described in detail.

References

Sep 1, 1994·Human Gene Therapy·M C LeavittF Wong-Staal
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·C WoffendinG J Nabel
Jan 1, 1993·Methods in Enzymology·J C SanfordJ A Russell
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·C WoffendinG J Nabel

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