Development of Molecular Markers Linked to Powdery Mildew Resistance Gene Pm4b by Combining SNP Discovery from Transcriptome Sequencing Data with Bulked Segregant Analysis (BSR-Seq) in Wheat

Frontiers in Plant Science
Peipei WuHongjie Li

Abstract

Powdery mildew resistance gene Pm4b, originating from Triticum persicum, is effective against the prevalent Blumeria graminis f. sp. tritici (Bgt) isolates from certain regions of wheat production in China. The lack of tightly linked molecular markers with the target gene prevents the precise identification of Pm4b during the application of molecular marker-assisted selection (MAS). The strategy that combines the RNA-Seq technique and the bulked segregant analysis (BSR-Seq) was applied in an F2:3 mapping population (237 families) derived from a pair of isogenic lines VPM1/7∗Bainong 3217 F4 (carrying Pm4b) and Bainong 3217 to develop more closely linked molecular markers. RNA-Seq analysis of the two phenotypically contrasting RNA bulks prepared from the representative F2:3 families generated 20,745,939 and 25,867,480 high-quality read pairs, and 82.8 and 80.2% of them were uniquely mapped to the wheat whole genome draft assembly for the resistant and susceptible RNA bulks, respectively. Variant calling identified 283,866 raw single nucleotide polymorphisms (SNPs) and InDels between the two bulks. The SNPs that were closely associated with the powdery mildew resistance were concentrated on chromosome 2AL. Among the 84 variants th...Continue Reading

References

Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M A Saghai-MaroofR W Allard
Nov 10, 2001·Annual Review of Phytopathology·S H HulbertQ Sun
May 17, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ch SingrünV Mohler
Oct 31, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·C AsnaghiA D'Hont
Feb 6, 2004·The Plant Journal : for Cell and Molecular Biology·Nabila YahiaouiBeat Keller
Feb 27, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Z-Q MaS-H Cheng
Apr 7, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·C KuleungI Dweikat
Feb 22, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ronghua ZhouJizeng Jia
Jun 6, 2008·International Journal of Plant Genomics·P K GuptaJ Kumar
Sep 3, 2008·Science in China. Series C, Life Sciences·Yuanlin DuanYongbiao Xue
Sep 27, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yuanfeng HaoHonggang Wang
Nov 6, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Toi J TsiloJames A Anderson
Nov 19, 2008·Genetics·Randy LambreghtsJennifer J Loros
Apr 24, 2009·Journal of Experimental Botany·P R Shewry
May 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Navreet K BhullarBeat Keller
Apr 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Aizhong CaoPeidu Chen
Jul 15, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hongqi MaZhengqiang Ma
May 16, 2012·PloS One·Sanzhen LiuPatrick S Schnable
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Oct 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sihai YangDacheng Tian
Jul 1, 1996·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·F J Zeller, S L Hsam
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Dec 24, 2014·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yuanfeng HaoJerry Johnson
Jul 17, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ruiqi ZhangPeidu Chen
Jul 19, 2016·The New Phytologist·Simon G Krattinger, Beat Keller
Nov 1, 2016·Genome Biology·Javier Sánchez-MartínBrande B H Wulff

❮ Previous
Next ❯

Citations

Nov 2, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Chong TanHui Feng
Nov 7, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Wei HuaChengdao Li
Jan 4, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Awais RasheedZhonghu He
Jan 18, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xiaomin LiuJ Scott McElroy
Feb 6, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Peipei WuHongjie Li
Jan 4, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Huagang HeShanying Zhu

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-Seq
BSR-Seq
Seq
PCR

Software Mentioned

Blast
Trimmomatic
STAR
HaplotypeCaller
/ Exp
GATK
SAS
EnsemblPlants
Mapmaker

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved