Development of Molecularly Imprinted Polymers To Block Quorum Sensing and Inhibit Bacterial Biofilm Formation

ACS Applied Materials & Interfaces
Luyao MaXiaonan Lu

Abstract

Bacterial biofilms are responsible for most clinical infections and show increased antimicrobial resistance. In this study, molecularly imprinted polymers (MIPs) were developed to specifically capture prototypical quorum sensing autoinducers [i.e., N-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-C12AHL)], interrupt quorum sensing, and subsequently inhibit biofilm formation of Pseudomonas aeruginosa, an important human nosocomial pathogen. The synthesis of MIPs was optimized by considering the amount and type of the functional monomers itaconic acid (IA) and 2-hydroxyethyl methacrylate (HEMA). IA-based MIPs showed high adsorption affinity toward 3-oxo-C12AHL with an imprinting factor of 1.68. Compared to IA-based MIPs, the adsorption capacity of HEMA-based MIPs was improved fivefold. HEMA-based MIPs significantly reduced biofilm formation (by ∼65%), whereas biofilm suppression by IA-based MIPs was neutralized because of increased bacterial attachment. The developed MIPs represent promising alternative biofilm intervention agents that can be applied to surfaces relevant to clinical settings and food processing equipment.

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Citations

Jun 20, 2019·Polymers·Bernadette-Emőke Teleky, Dan Cristian Vodnar
Dec 24, 2018·International Journal of Molecular Sciences·Alexandra Muñoz-BonillaMarta Fernández-García
Apr 28, 2020·Critical Reviews in Analytical Chemistry·Shabi Abbas Zaidi
Feb 26, 2019·Frontiers in Cellular and Infection Microbiology·Osmel Fleitas MartínezOctavio Luiz Franco
Feb 24, 2019·Bioorganic & Medicinal Chemistry Letters·Shixin Fa, Yan Zhao
Jul 31, 2020·Biosensors & Bioelectronics·Maha AlafeefDipanjan Pan

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