Development of multilayered cell-hydrogel composites using an acoustic focusing technique.

Biotechnology Progress
Jason P MazzoccoliPeter N Pintauro

Abstract

Multilayered composites, composed of mammalian cells arranged in a hydrogel, have been prepared using an acoustic focusing technique. Acoustic focusing is a simple, nonchemical technique that allows for the fast arrangement of cells in matrices where the control of cell geometry is beneficial. Breast cancer cells (MDA-MB231) were dispersed in a 30 wt % solution of poly(ethylene glycol) diacrylate (PEGDA) of molecular weight 400 at a density of 5 x 10(6) cells/mL of PEGDA solution. An ultrasonic field was used to organize the cells before polymerization of PEGDA. Disk-shaped hydrogel composites, typically 1 cm in diameter and 2-mm thick were prepared based on a PEGDA solution volume of 130 microL. At an acoustic frequency of 2.32 MHz, composites having cells positioned within concentric cylindrical shells interspersed with zones of cell-free hydrogel were produced. The cells were located in annuli approximately 80-microm thick and about 300 microm apart. The structure and viability of the cells within these constructs were studied using a fluorescent LIVE/DEAD assay. The viability of the cells was on the order of 50%. For the conditions used in this study, cell death was primarily attributed to exposure of cells to the PEGDA sol...Continue Reading

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Citations

Aug 3, 2013·Advanced Drug Delivery Reviews·Helen MulvanaMartyn Hill
Aug 28, 2021·Micromachines·Soheila Shabaniverki, Jaime J Juárez

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