Development of 'multiresistance rice' by an assembly of herbicide, insect and disease resistance genes with a transgene stacking system.

Pest Management Science
Chuanxu LiYongjun Lin

Abstract

Weeds, diseases and pests pose serious threats to rice production and cause significant economic losses. Cultivation of rice varieties with resistance to herbicides, diseases and pests is believed to be the most economical and environmentally friendly method to deal with these problems. In this study, a highly efficient transgene stacking system was used to assembly the synthetic glyphosate-tolerance gene (I. variabilis-EPSPS*), lepidopteran pest resistance gene (Cry1C*), brown planthopper resistance genes (Bph14* and OsLecRK1*), bacterial blight resistance gene (Xa23*) and rice blast resistance gene (Pi9*) onto a transformable artificial chromosome vector. The construct was transferred into ZH11 (a widely used japonica rice cultivar Zhonghua 11) via Agrobacterium-mediated transformation and 'multiresistance rice' (MRR) with desirable agronomic traits was obtained. The results showed that MRR had significantly improved resistance to glyphosate, borers, brown planthopper, bacterial blight and rice blast relative to the recipient cultivar ZH11. Besides, under the natural occurrence of pests and diseases in the field, the yield of MRR was significantly higher than that of ZH11. A multigene transformation strategy was employed to s...Continue Reading

References

Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Nov 27, 1998·Nature Biotechnology·L ChenC M Fauquet
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·K DattaS K Datta
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Li LinBaojian Li
Dec 16, 2004·Molecular Plant-microbe Interactions : MPMI·Anjali S Iyer, Susan R McCouch
Sep 28, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hao ChenQifa Zhang
Dec 6, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Zhaohui ChuShiping Wang
May 20, 2006·The Plant Journal : for Cell and Molecular Biology·Xuewei ChenLihuang Zhu
Jul 13, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yiwen DengZuhua He
Aug 26, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yi XiangShiping Wang
Apr 5, 2007·Nature Protocols·Ed Southern
Sep 22, 2007·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jinling LiuGuoliang Wang
Oct 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Qifa Zhang
Feb 15, 2008·Pest Management Science·Stephen O Duke, Stephen B Powles
Feb 4, 2009·Trends in Biotechnology·Pari Skamnioti, Sarah J Gurr
May 30, 2009·Pest Management Science·Rongjian YeYongjun Lin
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Bo DuGuangcun He
Dec 15, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Bin YuanQinghua Pan
Jan 14, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Philippa J Barrell, Anthony J Conner
Jan 22, 2011·The Plant Journal : for Cell and Molecular Biology·Yudai OkuyamaRyohei Terauchi
May 31, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Lixia HuaQinghua Pan
Nov 28, 2012·Molecular Biology Reports·G MiahM A Latif
Apr 7, 2015·Comptes rendus biologies·Fatah A TanweerMohammad A Latif
Jun 18, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Suk-Man KimKshirod K Jena
Oct 2, 2015·The Plant Journal : for Cell and Molecular Biology·Mathilde HutinBoris Szurek
Nov 15, 2015·Plant Science : an International Journal of Experimental Plant Biology·Ranjith K EllurAshok K Singh

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.