PMID: 15376531Sep 21, 2004Paper

Development of multispecies algal bioassays using flow cytometry

Environmental Toxicology and Chemistry
Natasha M FranklinRichard P Lim

Abstract

Multispecies algal bioassays, suitable for assessing copper toxicity, were developed with three marine (Micromonas pusilla, Phaeodactylum tricornutum, and Heterocapsa niei) and three freshwater (Microcystis aeruginosa, Pseudokirchneriella subcapitata, and Trachelomonas sp.) microalgae. Flow cytometry was used to separate and count algal signals based on pigment fluorescence and cell size. Species were mixed together on the basis of equivalent surface areas to avoid the confounding effect on toxicity of increased biomass for metal binding. Under control conditions (no added copper), M. pusilla growth was inhibited in the presence of the other marine microalgae compared to single-species tests, while the opposite was true (i.e., growth stimulation) for M. aeruginosa and P. subcapitata in freshwater mixtures. Competition for nutrients, including CO2, and algal exudate production may account for these effects. Interactions between microalgal species also had a significant effect on copper toxicity to some species. In freshwater multispecies bioassays, the toxicity of copper to Trachelomonas sp. was greater in the presence of other species, with copper concentrations required to inhibit growth (cell division) rate by 50% (72-h [IC50...Continue Reading

Citations

Apr 1, 2014·Aquatic Toxicology·Paulo de MoraisM Teresa S D Vasconcelos
Mar 19, 2013·Bulletin of Environmental Contamination and Toxicology·Ralph Alquezar, Amie Anastasi
Jan 25, 2016·Environmental Science and Pollution Research International·Xiaoxiong WangXiaojun Wang
Oct 3, 2008·Marine Environmental Research·M Teresa S D Vasconcelos, M Fernanda C Leal
Oct 16, 2015·Aquatic Toxicology·Brad M AngelSimon C Apte
Oct 12, 2010·Environmental Toxicology and Chemistry·Matilde Moreira-SantosRui Ribeiro
Aug 24, 2013·Environmental Toxicology and Chemistry·Hui-Ling Ou-YangFu-Liu Xu
Nov 11, 2006·Aquatic Toxicology·Anne-Hélène Le JeuneChristian Amblard
Dec 22, 2005·Ecotoxicology and Environmental Safety·Yang YuXiaoli Shi
Aug 15, 2009·Aquatic Toxicology·Carmen RiobooAngeles Cid
Sep 3, 2018·The Science of the Total Environment·E Bautista-ChamizoI Riba
Dec 30, 2018·Ecotoxicology and Environmental Safety·Giulia CheloniVera I Slaveykova
Jul 11, 2020·Chemosphere·Sreejith Kottuparambil, Susana Agusti
Nov 9, 2021·The Science of the Total Environment·David A SpadaroJenny L Stauber

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