Development of naked growing mouse oocytes in vitro

The Journal of Experimental Zoology
R BachvarovaA Tejblum

Abstract

Half grown oocytes were released from follicles of 7-day-old mouse ovaries during overnight culture. Naked oocytes free of attached follicular cells were cultured in MEM plus 10% fetal calf serum over the developing monolayer of ovarian cells. Oocytes increased in diameter for a period of at least a week at 1.6 micron per day, or 70% of the in vivo growth rate. The ultrastructure of cytoplasmic organelles in cultured growing oocytes was normal. During the second week, about 35% underwent spontaneous fragmentation, and at least 10-20% resumed meiotic maturation. We conclude that a significant fraction of naked oocytes reached a functional state comparable to that of normal full grown oocytes at a rate approaching the in vivo rate. Free oocytes with attached cells grew to a larger average diameter than naked oocytes, and incorporated 3H-leucine 75% more rapidly. Naked oocytes cultured in the absence of ovarian cells did not grow, and died when pyruvate was omitted from the medium. Naked oocytes did not grow when cultured over primary mouse fibroblasts, L-cells, CHO cells, or hepatoma cells. Naked oocytes separated from the monolayer of ovarian cells by a 0.7 mm layer of agar grew at 60% of the rate of control oocytes.

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·N Dekel, W H Beers
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R A Wallace, Z Misulovin
Oct 15, 1977·Developmental Biology·J J Eppig
Nov 1, 1977·The Journal of Experimental Zoology·M M Baran, R Bachvarova
Jul 1, 1978·The Journal of Cell Biology·N B GilulaW H Beers
Nov 1, 1976·The Journal of Cell Biology·E Anderson, D F Albertini
May 6, 1976·Nature·P M Wassarman, G E Letourneau
Aug 1, 1976·The Journal of Experimental Zoology·C L JahnR Bachvarova
Apr 1, 1975·Journal of Reproduction and Fertility·J DullaartM Ryle
Jun 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·K V HonnW Chavin
Jun 1, 1976·Developmental Biology·R A Sorensen, P M Wassarman
Jun 1, 1970·Biology of Reproduction·L Zamboni
Dec 1, 1971·Experimental Cell Research·G D BurkholderT A Okada
Apr 1, 1974·The American Journal of Anatomy·Y H Kang
Sep 1, 1969·Acta Endocrinologica·T Pedersen
Jun 1, 1970·Acta Endocrinologica·T Pedersen
May 1, 1968·Fertility and Sterility·T Fainstat
Dec 1, 1968·Journal of Reproduction and Fertility·T Pedersen, H Peters
Nov 1, 1968·Journal of Reproduction and Fertility·R P Donahue, S Stern

❮ Previous
Next ❯

Citations

Dec 1, 1992·Molecular Reproduction and Development·T C WuY J Wan
Jan 1, 1982·Chromosoma·R BachvarovaR S Chaganti
Apr 1, 1984·Developmental Biology·R CanipariF Mangia
Jul 1, 1992·Developmental Biology·D Wickramasinghe, D F Albertini
Jul 1, 1985·Cell Differentiation·J Fulka
Oct 20, 2011·The Journal of Biological Chemistry·Chia Lin ChangSheau Yu Teddy Hsu
Jul 8, 2000·Obstetrical & Gynecological Survey·C Yap
Aug 14, 2008·Physiological Genomics·Young S LeeCatherine A Vandevoort
Mar 14, 2013·BioMed Research International·Katarzyna Kossowska-Tomaszczuk, Christian De Geyter
Jun 26, 2007·The Journal of Reproduction and Development·Azusa InoueFugaku Aoki
Feb 18, 2004·The Journal of Reproduction and Development·Shoichiro SenbonTakashi Miyano
Jan 1, 1988·Annals of the New York Academy of Sciences·J J EppigA C Schroeder
Apr 18, 2006·Fertility and Sterility·Jean Clair SadeuJohan Smitz
Aug 2, 2006·Journal of the Society for Gynecologic Investigation·Xiao ZhangYing-Lu Guo
Sep 1, 1989·Gamete Research·S A DanielR E Gore-Langton
May 1, 1982·The Journal of Experimental Zoology·G KaplanR Bachvarova
Jun 1, 1987·The Journal of Experimental Zoology·R BuccioneR Colonna
Jul 12, 2008·Reproductive Biomedicine Online·Alessandra CamboniAnne Van Langendonckt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.