Development of next generation of therapeutic IFN-α2b via genetic code expansion

Acta Biomaterialia
Bo ZhangDemin Zhou

Abstract

With the aim to overcome the heterogeneity associated with marketed IFN-α2b PEGylates and optimize the size of the PEG moiety and the site of PEGylation, we develop a viable and facile platform through genetic code expansion for PEGylation of IFN-α2b at any chosen site(s). This approach includes site-specific incorporation of an azide-bearing amino acid into IFN-α2b followed by orthogonal and stoichiometric conjugation of a variety of PEGs via a copper-free click reaction. By this approach, only the chosen site(s) within IFN-α2b is consistently PEGylated under mild conditions, leading to a single and homogenous conjugate. Furthermore, it makes the structure-activity relationship study of IFN-α2b possible by which the opposite effects of PEGylation on the biological and pharmacological properties are optimized. Upon re-examination of the PEGylated IFN-α2b isomers carrying different sizes of PEG at different sites, we find mono-PEGylates at H34, A74 and E107 with a 20-, 10- and 10-kDa PEG moiety, respectively, have both higher biological activities and better PK profiles than others. These might represent the direction for development of the next generation of PEGylated IFN-α2b.

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Citations

Dec 22, 2016·Bioconjugate Chemistry·Imran Ozer, Ashutosh Chilkoti
Feb 28, 2019·Chemical Science·Karolina PeciakSteve Brocchini
Mar 24, 2021·Journal of the American Chemical Society·Miglena ManandharFloyd E Romesberg
Jun 5, 2018·Bioconjugate Chemistry·Yali HuHua Lu

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