Development of non-agglutination microarray blood grouping

Transfusion Medicine
Janine S RobbJuraj Petrik

Abstract

Microarray technology provides an opportunity to monitor multiple parameters simultaneously. High-throughput applications such as blood donation screening could greatly benefit from performing various tests on a single testing platform. Blood grouping represents one part of the donation testing complementing the screening for blood-borne pathogens. Blood group serology traditionally exploited agglutination as the detection method. In this investigation, we have adapted blood grouping reactions to a solid-phase microarray substrate in a non-agglutination reaction format as an initial step in the development of a combined microarray testing platform. We have investigated immobilization of proprietary antibodies on multiple surfaces and monitored their performance under various reaction conditions. For the first time, highly specific blood grouping has been achieved on a planar microarray using directly labelled erythrocytes or a secondary labelled reagent using fluorescent signal end point readout. We have also complemented microarray data with a label-free, surface plasmon resonance-based Biacore platform data and used the real time quantitative measurement to rank anti-A antibodies according to the strength of reaction with the...Continue Reading

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Citations

Mar 25, 2008·Journal of Immunoassay & Immunochemistry·Nichola O'LooneyColin J Campbell
Jan 19, 2010·Biologicals : Journal of the International Association of Biological Standardization·Juraj Petrik
Apr 27, 2007·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Juraj PetrikJerard Seghatchian
Nov 20, 2008·British Journal of Haematology·Neil D Avent
Aug 2, 2006·Transfusion Medicine·J Petrik
Jun 16, 2010·Transfusion·Chantal Fournier-WirthJoliette Coste
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Jan 11, 2019·Perspectives on Psychological Science : a Journal of the Association for Psychological Science·Melissa A KoenigJ Kiley Hamlin

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