Development of Non-proximate Probe Electrospray Ionization for Real-Time Analysis of Living Animal

Mass Spectrometry
Kentaro YoshimuraSen Takeda

Abstract

Ambient ionization mass spectrometry is one of the most challenging analytical tools in the field of biomedical research. We previously demonstrated that probe electrospray ionization mass spectrometry (PESI-MS) could potentially be used in the rapid diagnosis of cancer. Although this technique does not require a tedious sample pretreatment process, it was not possible for our previously reported setup to be applied to cases involving the direct sampling of tissues from living animal and large animal subjects, because there would not be enough room to accommodate the larger bodies juxtaposed to the ion inlet. To make PESI-MS more applicable for the real-time analysis of living animals, a long auxiliary ion sampling tube has been connected to the ion inlet of the mass spectrometer to allow for the collection of ions and charged droplets from the PESI source (hereafter, referred to as non-proximate PESI). Furthermore, an additional ion sampling tube was connected to a small diaphragm pump to increase the uptake rate of air carrying the ions and charged droplets to the ion inlet. This modification allows for the extended ion sampling orifice to be positioned closer to the specimens, even when they are too large to be placed inside...Continue Reading

References

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Apr 15, 2005·Analytical Chemistry·Robert B CodyH Dupont Durst
Aug 22, 2007·Rapid Communications in Mass Spectrometry : RCM·Kenzo HiraokaShigeo Suzuki
Mar 24, 2009·Journal of Mass Spectrometry : JMS·Kentaro YoshimuraSen Takeda
Aug 18, 2009·Journal of Mass Spectrometry : JMS·Lee Chuin ChenKenzo Hiraoka
Aug 5, 2010·Analytical Chemistry·Julia BalogZoltan Takats
Jul 12, 2011·Analytical Biochemistry·Kentaro YoshimuraSen Takeda
Oct 21, 2011·Journal of Mass Spectrometry : JMS·Mridul Kanti MandalKenzo Hiraoka
Feb 24, 2012·Journal of Mass Spectrometry : JMS·Sandilya GarimellaZheng Ouyang
Aug 1, 2012·Journal of the American Society for Mass Spectrometry·Kentaro YoshimuraSen Takeda
Jul 19, 2013·Science Translational Medicine·Júlia BalogZoltán Takáts

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Citations

Jan 24, 2019·Endocrine Connections·Mélanie RouleauFrédéric Pouliot
Aug 31, 2017·Mass Spectrometry·Lee Chuin ChenKenzo Hiraoka
Jun 16, 2017·The Analyst·Lee Chuin ChenKenzo Hiraoka
Aug 14, 2021·JGH Open : an Open Access Journal of Gastroenterology and Hepatology·Seiichiro FukuharaTakanori Kanai

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