Development of novel cationic microemulsion as parenteral adjuvant for influenza vaccine

Asian Journal of Pharmaceutical Sciences
Sakalanunt LamaisakulGarnpimol Ritthidej

Abstract

Squalene-based oil-in-water (O/W) emulsions have been used as effective and safe adjuvants in approved influenza vaccines. However, there are concerns regarding the safety and side effects of increasing risk of narcolepsy. In present study, novel O/W microemulsions (MEs) containing wheat germ oil, D-alpha tocopheryl polyethylene glycol 1000 succinate (TPGS) and Cremophor EL (CreEL) or Solutol HS15 were formulated with/without a cationic surfactant, cetyltrimethylammonium bromide (CTAB) and then sterilized by autoclaving. Their physical properties and biological efficacies were evaluated. The results demonstrated that autoclaving reduced the droplet size to ∼20 nm with narrow size distributions resulting in monodisperse systems with good stability up to 3 years. Hemolytic activity, viscosity, pH, and osmolality were appropriate for parenteral use. Bovine serum albumin (BSA), a model antigen, after mixing with MEs retained the protein integrity, assessed by SDS-PAGE and CD spectroscopy. Greater percentages of 28SC cell viability were observed from CreEL-based MEs. Uptake of FITC-BSA-MEs increased with the increasing concentration of CTAB confirmed by CLSM images. Furthermore, cationic CreEL-based MEs could induce Th1 cytokine syn...Continue Reading

Citations

Sep 15, 2020·Drug Delivery and Translational Research·Karoll Moangella Andrade de AssisBolívar Ponciano Goulart de Lima Damasceno

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Methods Mentioned

BETA
ELISA
dynamic light scattering
electrophoresis
enzyme-linked immunosorbent assay
flow cytometry
biopsy

Software Mentioned

CaseViewer
SPSS statistics
CreEL

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