Development of novel formulations containing Lysozyme and Lactoferrin and evaluation of antibacterial effects on Mutans Streptococci and Lactobacilli

Archives of Oral Biology
K Tonguc-AltinGüven Kulekci

Abstract

The aim of this study was to determine the antibacterial effect of different formulations containing Lysozyme and Lactoferrin and drug delivery system as well as poloxamer 407 with the trade name of Pluronic F-127 and/or freeze dried liposome containing DOTAP [freeze dried Liposomal DOTAP] on Streptococcus sobrinus, Streptococcus mutans and Lactobacillus acidophilus in comparison with 0.2% chlorhexidine. The antibacterial effect was assessed by determining the minimum inhibitory concentrations (MIC) for the study and control groups on Streptococcus sobrinus, Streptococcus mutans and Lactobacillus acidophilus. The amounts of biofilm formation accumulation of Mutans Streptococci for 24h on sterile hydroxyapatite discs after application of different formulations were evaluated. The different formulations studied were: (1) Sorensen's Buffer Solution, (2) a gel formulation containing only poloxamer 407, (3) Lysozyme and Lactoferrin dissolved in Sorensen's Buffer Solution, (4) poloxamer 407 combined with the third formulation, (5) Freeze dried Liposomal DOTAP dissolved in Sorensen's Buffer Solution, (6) Freeze dried Liposomal DOTAP combined with poloxamer 407 dispersed in Sorensen's Buffer Solution, (7) Freeze dried Liposomal DOTAP c...Continue Reading

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Citations

Mar 13, 2016·Biotechnology and Applied Biochemistry·Małgorzata PleszczyńskaJanusz Szczodrak
Mar 19, 2020·International Journal of Molecular Sciences·Katarzyna Niemirowicz-LaskowskaRobert Bucki
Aug 10, 2021·Advanced Drug Delivery Reviews·Chelsea R ThornNicky Thomas
Dec 30, 2021·Vestnik otorinolaringologii·A V GurovA V Muzhichkova

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