Development of novel human in vitro vascularized adipose tissue model with functional macrophages

Cytotechnology
Outi HuttalaTimo Ylikomi

Abstract

Inflammation has been proven significant factor in development of type 2 diabetes. So far, most of the adipose tissue related research has been performed in animals, mainly rodent models. The relevance of translation of animal results to humans is questionable. However, in vitro model with relevant human cell source, such as human adipose tissue stromal cells (hASC), can be developed and should be utilized for human adipose tissue research. We developed in vitro models of human adipose tissue utilizing hASC, endothelial cells and monocytes/macrophages. By isolating endothelial cells and macrophages from same adipose tissue as hASC, we were able to provide method for constructing personalized models of adipose tissue. With these models, we studied the effect of macrophages on adipogenesis and protein secretion, with and without vasculature. The models were analyzed for immunocytochemical markers, cell number, triglyceride accumulation and protein secretion. We found that lipid accumulation was greater in adipocytes in the presence of macrophages. Interferon gamma increased this difference between adipocyte culture and Adipocyte-Macrophage co-culture. Protein secretion was affected more by macrophages when vasculature was not pre...Continue Reading

References

Feb 11, 1983·Journal of Immunological Methods·R AndreesenG W Löhr
Aug 26, 1998·The Journal of Clinical Endocrinology and Metabolism·P DandonaT Wadden
Jul 21, 2000·The Journal of Clinical Investigation·G I Shulman
Feb 24, 2001·Nature·C M SteppanM A Lazar
Jul 24, 2001·JAMA : the Journal of the American Medical Association·A D PradhanP M Ridker
Jun 11, 2002·Endocrine Journal·S ShigematsuK Hashizume
Jun 18, 2002·Nature Medicine·Norikazu MaedaYuji Matsuzawa
Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aihua LiRakesh K Singh
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Takeshi ImaiDaniel Metzger
Jul 23, 2005·International Journal of Clinical Practice. Supplement·R A DeFronzo
Nov 22, 2005·Life Sciences·Wayne Huey-Herng SheuChing-Hwa Yang
Dec 3, 2005·Nature Reviews. Immunology·Siamon Gordon, Philip R Taylor
Jan 21, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Blair WagonerRuth B S Harris
Apr 8, 2006·Immunity & Ageing : I & a·Massimo MariottiJeanette A M Maier
Aug 24, 2006·American Journal of Physiology. Endocrinology and Metabolism·Carey N LumengAlan R Saltiel
Jan 12, 2008·Annual Review of Medicine·Janet Woodcock, Raymond Woosley
Jul 29, 2008·Biochemical and Biophysical Research Communications·Jianping Ye
Jul 29, 2008·Circulation Research·Viviane Zorzanelli RochaPeter Libby
Jan 29, 2009·The Journal of Clinical Endocrinology and Metabolism·Eléonore MaurySonia M Brichard
Feb 12, 2009·Tissue Engineering. Part a·Jennifer H KangDavid L Kaplan
Feb 17, 2010·Endocrine Reviews·Mary-Elizabeth Patti, Silvia Corvera
Apr 3, 2010·PLoS Medicine·H Bart van der WorpMalcolm R Macleod
May 22, 2010·Tissue Engineering. Part a·Stephanie Merfeld-ClaussDmitry O Traktuev
Sep 3, 2011·Clinical and Experimental Pharmacology & Physiology·Jianping Ye, Jeffery M Gimble
Sep 10, 2011·Tissue Engineering. Part a·Jertta-Riina SarkanenTimo Ylikomi
Oct 11, 2011·Nature Reviews. Immunology·Ajay ChawlaY P Sharon Goh
Oct 15, 2011·Nature Reviews. Immunology·Peter J Murray, Thomas A Wynn
Nov 9, 2011·Translational Research : the Journal of Laboratory and Clinical Medicine·Pilar Codoñer-FranchEulalia Alonso-Iglesias
Mar 6, 2012·Metabolism: Clinical and Experimental·Robert W O'RourkeDaniel L Marks
Jul 21, 2012·Nature Immunology·Thirumala-Devi Kanneganti, Vishwa Deep Dixit
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Junhee SeokUNKNOWN Inflammation and Host Response to Injury, Large Scale Collaborative Research Program
May 15, 2013·Archives of Medical Science : AMS·Marisa CoelhoRuben Fernandes

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Citations

Feb 3, 2021·Tissue Engineering. Part a·Trivia FrazierJeffrey M Gimble
Aug 7, 2021·Frontiers in Endocrinology·Emma BokobzaNicolas Chevalier

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Methods Mentioned

BETA
scraping
Protein Assay
ELISA

Software Mentioned

GraphPadPrism
Cell
Adobe Photoshop
GraphPad

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