Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium

Parasites & Vectors
Bonnie L WebsterJoanne P Webster

Abstract

Human urogenital schistosomiasis caused by Schistosoma haematobium is widely distributed across Africa and is increasingly targeted for control and regional elimination. The development of new high-throughput, cost-effective molecular tools and approaches are needed to monitor and evaluate the impact of control programs on the parasite populations. Microsatellite loci are genetic markers that can be used to investigate how parasite populations change over time and in relation to external influences such as control interventions. Here, 18 existing S. haematobium microsatellite loci were optimised to enable simultaneous amplification across two novel multiplex microsatellite PCR's, each containing nine loci. Methods were developed for the cost effective and rapid processing and microsatellite analysis of S. haematobium larval stages stored on Whatman-FTA cards and proved robust on miracidia and cercariae collected from Zanzibar and Niger. The development of these novel and robust multiplex microsatellite assays, in combination with an improved protocol to elute gDNA from Whatman-FTA fixed schistosome larval stages, enables the high-throughput population genetic analysis of S. haematobium. The molecular resources and protocols des...Continue Reading

References

Mar 1, 2012·International Journal for Parasitology·Lynn MeursKatja Polman
Sep 5, 2012·Parasites & Vectors·Aidan M EmeryDavid Rollinson
Nov 13, 2012·PLoS Neglected Tropical Diseases·Bonnie L WebsterDavid Rollinson

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Citations

May 14, 2020·PLoS Neglected Tropical Diseases·Suzan C M TrienekensPoppy H L Lamberton
May 14, 2020·The American Journal of Tropical Medicine and Hygiene·Joanne P WebsterDavid Rollinson
May 29, 2018·Parasitology·Winka Le Clec'hTimothy J C Anderson
May 23, 2020·Emerging Infectious Diseases·Stefano CatalanoJoanne P Webster
Jan 27, 2019·Parasites & Vectors·Félicité Flore Djuikwo-TeukengJérôme Boissier
Mar 6, 2019·International Journal for Parasitology. Parasites and Wildlife·Stefano CatalanoJoanne P Webster
Jan 25, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·O ReyJ Boissier
Aug 12, 2021·Clinical Microbiology Reviews·Madeleine J RogersCatherine A Gordon

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Methods Mentioned

BETA
PCRs
PCR
electrophoresis
genotyping

Software Mentioned

GenAlEx
Geneious
Micro
Checker

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