Development of Novel Photosensitizer Using the Buddleja officinalis Extract for Head and Neck Cancer

Evidence-based Complementary and Alternative Medicine : ECAM
Hyejoung ChoOkjoon Kim

Abstract

Photodynamic therapy (PDT) is generally safer and less invasive than conventional strategies for head and neck cancer treatment. However, currently available photosensitizers have low selectivity for tumor cells, and the burden and side effects are so great that research is needed to develop safe photosensitizers. In this study, it was confirmed that the Buddleja officinalis (BO) extract, used in the treatment of inflammation and vascular diseases, shows fluorescence when activated by LED light, and, based on this, we aimed to develop a new photosensitive agent suitable for PDT. MTT, Diff-Quick® staining, and DCF-DA were performed to measure the effects of treating head and neck cancer cells with BO extract and 625 nm LED light (BO-PDT). Cell cycle, TUNEL, and western blot assays, as well as acridine orange staining, were performed to explore the mechanism of BO-PDT-induced cell death. We found that when the BO extract was irradiated with 625 nm LED light, it showed sufficient fluorescence and stronger intracellular toxicity and ROS effect than the currently commercially available hematoporphyrin. BO-PDT resulted in a decrease of mTOR activity that was correlated with an increase in the levels of ATG5, beclin-1, and LC3-II, whi...Continue Reading

References

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Mar 23, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jian-Feng XueXiao-Lin Li

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Citations

Jul 17, 2020·OncoTargets and Therapy·Yang-Jie HuShui-Hong Zhou
Oct 30, 2021·Photodiagnosis and Photodynamic Therapy·Mengyu LuoLing Kang

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Methods Mentioned

BETA
Fluorescence
enzyme-linked immunosorbent assay
flow cytometry
confocal microscopy
optical microscopy
electrophoresis

Software Mentioned

Image J

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