Development of nuclear microsatellite markers for the fonio, Digitaria exilis (Poaceae), an understudied West African cereal

American Journal of Botany
Adeline BarnaudClaire Billot

Abstract

We developed nuclear microsatellite primers to explore the genetic diversity, population genetic structure, and evolutionary history of the fonio (Digitaria exilis), an understudied cereal cultivated in West Africa. We used a microsatellite-enriched library approach to isolate and characterize 38 nuclear primer pairs (31 di-, five tri-, and two tetranucleotide repeats), of which 21 were polymorphic and exhibited a clear pattern in 36 accessions from West Africa. The number of alleles per locus ranged from two to 22, with a mean of 4.71, and expected heterozygosity ranged from 0.03 to 0.93. The developed set of 21 polymorphic SSR markers will provide tools for population and evolutionary genetics studies of the cultivated fonio.

References

Aug 30, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·H Adoukonou-SagbadjaW Friedt
Dec 1, 2007·BMC Bioinformatics·Alexis DereeperManuel Ruiz
Jan 1, 2008·Molecular Ecology Resources·François Rousset

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Citations

Sep 10, 2020·Nature Communications·Michael AbroukSimon G Krattinger

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