Development of oligonucleotide-based antagonists of Ebola virus protein 24 inhibiting its interaction with karyopherin alpha 1

Organic & Biomolecular Chemistry
Keisuke TanakaSatoshi Obika

Abstract

The investigation of protein-protein interactions (PPIs) and the preparation of antagonists are important for determining whether certain proteins are suitable medical targets. In the present study, we used the capillary electrophoresis-systematic evolution of ligands by exponential enrichment to generate natural and artificial nucleic acid aptamers targeting Ebola virus protein 24 (eVP24), demonstrating that artificial aptamers, synthesised utilising a uridine analogue with an adenine residue at its C5 position, exhibited activities exceeding those of natural ones. To confirm the functionality of the as-prepared aptamers, their abilities to inhibit the PPIs of eVP24 were determined by capillary electrophoresis and bio-layer interferometry, and the obtained results unambiguously demonstrated that these aptamers interacted with the functional site of eVP24 and were thus good antagonists.

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Citations

May 6, 2020·Antimicrobial Agents and Chemotherapy·Elisa FanunzaEnzo Tramontano
May 1, 2021·International Journal of Molecular Sciences·Tae-Hyeong Kim, Seong-Wook Lee
Dec 3, 2021·Journal of Medicinal Chemistry·Xiao-Fei ChenZifeng Yang

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Methods Mentioned

BETA
PCR
SELEX
electrophoresis
bio-layer interferometry
chips
chip
CE-SELEX

Software Mentioned

Octet Data Analysis
RaPID
Karat
FAQCs

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