Abstract
In recent years, protein chemistry tends inexorably toward the analysis of more complex proteins, proteoforms, and posttranslational protein modifications. Although mass spectrometry developed quite fast correspondingly, sample preparation and separation of these analytes is still a major issue and quite challenging. For many years, electrophoresis seemed to be the method of choice; nonetheless its variance is limited to parameters such as size and charge. When taking a look at traditional (thin-layer) chromatography, further parameters such as polarity and different mobile and stationary phases can be utilized. Further, possibilities of detection are manifold compared to electrophoresis. Similarly, two-dimensional separation can be also performed with thin-layer chromatography (TLC). As the revival of TLC developed enormously in the last decade, it seems to be also an alternative to use high performance thin-layer chromatography (HPTLC) for the separation of proteins. The aim of this study was to establish an HPTLC separation system that allows a separation of protein mixtures over a broad polarity range, or if necessary allowing to modify the separation with only few steps to improve the separation for a specific scope. Sever...Continue Reading
References
Nov 1, 1966·Analytical Biochemistry·S Katz, A Lewis
Oct 31, 1998·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·K OutinenH Vuorela
Oct 25, 2000·Journal of Chromatography. a·C F Poole, N C Dias
Jan 8, 2003·Journal of Chromatographic Science·Sz Nyiredy
Oct 1, 1964·Journal of Chromatography·C J MORRIS
Dec 9, 2003·Amino Acids·E R Stadtman, R L Levine
Oct 7, 2006·Proteomics·Vitaly KochinJohn E Eriksson
May 8, 2007·Journal of Chromatography. a·Venkateswarlu PanchagnulaWayne F Patton
Jun 15, 2007·Journal of Separation Science·Tomasz Tuzimski
Feb 12, 2008·Analytical and Bioanalytical Chemistry·Sofie P PasilisSusanne Schorcht
Jun 20, 2008·Journal of Mass Spectrometry : JMS·Sofie P PasilisSusanne Schorcht
Feb 4, 2010·Proteomics·Lucie SokolovaUlrich Brandt
May 25, 2010·Journal of Chromatography. a·Gertrud Morlock, Wolfgang Schwack
Aug 25, 2010·The Journal of Cell Biology·Tobias C Walther, Matthias Mann
Oct 25, 2011·Molecular & Cellular Proteomics : MCP·Nagarjuna NagarajMatthias Mann
Nov 8, 2011·Journal of Chromatography. a·Tomasz Tuzimski
Jun 19, 2013·Electrophoresis·Dimitrios G PapasotiriouBjoern Meyer
Jul 25, 2013·Phytochemical Analysis : PCA·Kathrin TscherchSascha Rohn
Dec 20, 2013·Journal of Chromatography. a·Julia WannenmacherGertrud E Morlock
Jul 30, 2014·Analytical Chemistry·Gertrud E Morlock, Ines Klingelhöfer
Dec 3, 2014·Journal of Chromatography. a·Gertrud E Morlock
Citations
Dec 18, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lena MorschheuserSascha Rohn
May 26, 2016·Scientific Reports·Lena MorschheuserSascha Rohn
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jenny SpöttelSascha Rohn
Aug 9, 2021·Journal of Chromatography. a·Mascha TreblinSascha Rohn