Development of Patient-Derived Human Monoclonal Antibodies Against Nucleocapsid Protein of Severe Acute Respiratory Syndrome Coronavirus 2 for Coronavirus Disease 2019 Diagnosis

Frontiers in Immunology
Li ZhangFengcai Zhu


The pandemic caused by emerging Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) presents a global public health threat. Illustrating human antibody responding to viral antigen could potentially provide valuable information for basic research and clinical diagnosis. The antibody can be used as a complement to the viral detection for the rapid diagnosis of infected patients. Compared with spike protein (SP), nucleocapsid protein (NP) is normally conserved and highly immunogenic in many coronavirus members. As a major antigen, NP is a potential target for the diagnosis of SARS-CoV-2 infection. Here, we constructed a combinatorial fragment of antigen-binding (Fab)antibody phage library based on peripheral blood-derived from five coronavirus disease 2019 (COVID-19) infected donors. From the library, 159 Fab antibodies were obtained and identified by panning with NP. Among them, 16 antibodies were evaluated for their binding properties and epitopes recognition. Among these 16 antibodies, two well-paired antibodies were finally screened out for SARS-CoV-2 diagnosis by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) method. Our works may provide a potential resource for the clinical diagnosis of SARS-CoV...Continue Reading


Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C F BarbasS J Benkovic
Jul 1, 1996·Trends in Biotechnology·C F Barbas, D R Burton
Jun 25, 2004·The Journal of Infectious Diseases·Danny Tze Ming LeungPak Leong Lim
Nov 20, 2004·Emerging Infectious Diseases·Xiao-Yan CheKwok-Yung Yuen
Nov 9, 2005·Protein Expression and Purification·Alexsandra R M FavachoVera S Gouvea
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Heng ZhuMichael Snyder
Aug 12, 2014·Viruses·Ruth McBrideBurtram C Fielding
Oct 1, 2015·Emerging Microbes & Infections·Yixin ChenPatrick C Y Woo
Apr 12, 2016·Biotechnology Journal·Yuyu TanChaoyong J Yang
Nov 22, 2017·Frontiers of Medicine·Yujia JinTianlei Ying
Jan 25, 2020·The New England Journal of Medicine·Na ZhuUNKNOWN China Novel Coronavirus Investigating and Research Team
Jan 30, 2020·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Victor M CormanChristian Drosten
Mar 1, 2020·The Journal of Infection·Sijia TianJinjun Zhang
Mar 1, 2020·International Journal of Surgery·Catrin SohrabiRiaz Agha
Mar 20, 2020·International Journal of Health Services : Planning, Administration, Evaluation·Wim De Ceukelaire, Chiara Bodini
Mar 24, 2020·Nature Biotechnology·Cormac Sheridan
Apr 2, 2020·Journal of Clinical Microbiology·Wanbing LiuShangen Zheng
Apr 7, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Xun LiZuojiong Gong
Apr 28, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jiuxin QuLei Liu
May 3, 2020·Emerging Microbes & Infections·Baoqing SunLing Chen
May 18, 2020·Biochemical and Biophysical Research Communications·Weihong ZengTengchuan Jin
May 28, 2020·The New England Journal of Medicine·Eboni G Price-HaywoodLeonardo Seoane
Jun 9, 2020·BioRxiv : the Preprint Server for Biology·Qiaozhen YeKevin D Corbett
Jun 9, 2020·MedRxiv : the Preprint Server for Health Sciences·Peter D BurbeloJeffrey I Cohen
Jun 21, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sean M Cascarina, Eric D Ross
Jun 26, 2020·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Gannon Ck MakDominic Nc Tsang
Jun 27, 2020·BioRxiv : the Preprint Server for Biology·Jasmine CubukAlex S Holehouse

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Datasets Mentioned


Methods Mentioned

phage display
enzyme-linked immunosorbent assay
Protein Assay
Surface plasmon resonance

Software Mentioned

Biacore T200
GraphPad Prism

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