Development of Patient-Derived Human Monoclonal Antibodies Against Nucleocapsid Protein of Severe Acute Respiratory Syndrome Coronavirus 2 for Coronavirus Disease 2019 Diagnosis

Frontiers in Immunology
Li ZhangFengcai Zhu

Abstract

The pandemic caused by emerging Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) presents a global public health threat. Illustrating human antibody responding to viral antigen could potentially provide valuable information for basic research and clinical diagnosis. The antibody can be used as a complement to the viral detection for the rapid diagnosis of infected patients. Compared with spike protein (SP), nucleocapsid protein (NP) is normally conserved and highly immunogenic in many coronavirus members. As a major antigen, NP is a potential target for the diagnosis of SARS-CoV-2 infection. Here, we constructed a combinatorial fragment of antigen-binding (Fab)antibody phage library based on peripheral blood-derived from five coronavirus disease 2019 (COVID-19) infected donors. From the library, 159 Fab antibodies were obtained and identified by panning with NP. Among them, 16 antibodies were evaluated for their binding properties and epitopes recognition. Among these 16 antibodies, two well-paired antibodies were finally screened out for SARS-CoV-2 diagnosis by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) method. Our works may provide a potential resource for the clinical diagnosis of SARS-CoV...Continue Reading

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Citations

Mar 16, 2021·Emergent Materials·Praopim LimsakulItthipon Jeerapan
Jul 27, 2021·Frontiers in Cellular and Infection Microbiology·Kristian Daniel Ralph RothMichael Hust

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Datasets Mentioned

BETA
MT066176.1
MW116179
MW116210

Methods Mentioned

BETA
phage display
PCR
enzyme-linked immunosorbent assay
ELISA
Protein Assay
chip
Surface plasmon resonance
bronchoalveolar
lavage

Software Mentioned

Weblogo
FastTree
Excel
ClustalW
blast
MEGA
Biacore T200
DNAPLOT
GeneBank
GraphPad Prism

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