Development of PCR-Based Assays for Detecting Xanthomonas campestris pv. carotae, the Carrot Bacterial Leaf Blight Pathogen, from Different Substrates

Plant Disease
X Q MengR L Gilbertson

Abstract

Detection of the carrot bacterial leaf blight pathogen, Xanthomonas campestris pv. carotae, was achieved using polymerase chain reaction (PCR) along with primer pairs developed from sequences of cloned random amplified polymorphic DNA (RAPD) fragments. Primer pairs 3S and 9B directed the amplification of ∼350-bp and ∼900-bp (or ∼2 kb) DNA fragments, respectively, from genomic DNA of all known X. campestris pv. carotae strains tested, but not from that of 13 other X. campestris pathovars or other bacterial species, including yellow non-xanthomonad bacteria isolated from carrot tissues and seeds. In tests conducted with an extensive collection of X. campestris pv. carotae-like strains isolated from different substrates from California, Idaho, Oregon, Washington, and Canada, the 3S primer pair directed the amplification of the ∼350-bp target fragment from all strains. These results indicated that the 3S primer pair is highly specific for X. campestris pv. carotae detection. Using the 3S primer pair, PCR assays were developed for detection of X. campestris pv. carotae from colonies on agar media, carrot leaf and stem tissues, and seeds. These tests could be performed in a single day. The PCR-based seed assay detected X. campestris ...Continue Reading

Citations

May 4, 2007·Annual Review of Phytopathology·Ronald Gitaitis, Ronald Walcott
Aug 1, 2005·Plant Disease·Lindsey J du ToitGary Q Pelter
Mar 9, 2010·Applied and Environmental Microbiology·Isabelle Robène-SoustradeOlivier Pruvost
Jan 25, 2022·Molecular Plant Pathology·Nay C DiaJoël F Pothier

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