Development of polymorphic microsatellite loci in the perennial herb Hepatica nobilis var. japonica (Ranunculaceae)

Applications in Plant Sciences
Shinichiro KameokaHiroaki Setoguchi

Abstract

Microsatellite markers were developed and characterized in the vulnerable plant Hepatica nobilis var. japonica (Ranunculaceae) to investigate its genetic diversity, population structure, and gene flow. Fourteen microsatellite markers were developed. The number of alleles per locus ranged from one to 12, and the expected heterozygosity per locus ranged from 0.043 to 0.855. Eleven markers were successfully amplified in the cultivar 'Mego' from Japan. These microsatellite markers can be used to investigate the genetic diversity, population structure, and gene flow of H. nobilis var. japonica.

References

Apr 26, 2006·Journal of Plant Research·Chunlan L LianTaizo Hogetsu
Dec 8, 2006·Molecular Biology and Evolution·Marie-Pierre Chapuis, Arnaud Estoup

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Citations

Aug 26, 2016·Genetics and Molecular Biology·Maria Lucia Carneiro VieiraCarla de Freitas Munhoz
Dec 23, 2016·Journal of Plant Research·Shinichiro KameokaHiroaki Setoguchi

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

GenAlEx
STRUCTURE
GeneMapper
FSTAT

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