Development of Polymorphic Microsatellite Loci for Potato Wart from Next-Generation Sequence Data

Phytopathology
Marie-Claude GagnonGuillaume J Bilodeau

Abstract

Synchytrium endobioticum is the fungal agent causing potato wart disease. Because of its severity and persistence, quarantine measures are enforced worldwide to avoid the spread of this disease. Molecular markers exist for species-specific detection of this pathogen, yet markers to study the intraspecific genetic diversity of S. endobioticum were not available. Whole-genome sequence data from Dutch pathotype 1 isolate MB42 of S. endobioticum were mined for perfect microsatellite motifs. Of the 62 selected microsatellites, 21 could be amplified successfully and displayed moderate levels of polymorphism in 22 S. endobioticum isolates from different countries. Nineteen multilocus genotypes were observed, with only three isolates from Canada displaying identical profiles. The majority of isolates from Canada clustered genetically. In contrast, most isolates collected in Europe show no genetic clustering associated with their geographic origin. S. endobioticum isolates with the same pathotype displayed highly variable genotypes and none of the microsatellite markers correlated with a specific pathotype. The markers developed in this study can be used to assess intraspecific genetic diversity of S. endobioticum and allow track and tr...Continue Reading

References

Feb 5, 2000·Nature Biotechnology·M Schuelke
Dec 6, 2002·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Masashi ShimizuMitsuru Emi
May 22, 2004·Nature Reviews. Genetics·Hans Ellegren
Nov 26, 2004·Molecular Biology and Evolution·Haydar KaraogluWieland Meyer
Oct 20, 2007·Molecular Ecology·S Arnaud-HaondE A Serrão
Aug 19, 2008·Molecular Plant Pathology·Soledad Sacristán, Fernando García-Arenal
Apr 13, 2011·Molecular Ecology Resources·Lindsay V Clark, Marie Jasieniuk
Feb 8, 2014·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jude Ejikeme ObidiegwuChristiane Gebhardt

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Citations

Jul 23, 2018·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (EFSA PLH Panel)Vittorio Rossi

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