Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay

BMC Veterinary Research
Thi Ngan MaiSatoshi Sekiguchi

Abstract

Porcine epidemic diarrhoea (PED) is an emerging disease in pigs that causes massive economic losses in the swine industry, with high mortality in suckling piglets. Early identification of PED virus (PEDV)-infected herd through surveillance or monitoring strategies is necessary for mass control of PED. However, a common working diagnosis system involves identifying PEDV-infected animals individually, which is a costly and time-consuming approach. Given the above information, the thrusts of this study were to develop a real-time fluorescent reverse transcription loop-mediated isothermal amplification (RtF-RT-LAMP) assay and establish a pooled testing system using faecal sample to identify PEDV-infected herd. In this study, we developed an accurate, rapid, cost-effective, and simple RtF- RT-LAMP assay for detecting the PEDV genome targeting M gene. The pooled testing system using the RtF-RT-LAMP assay was optimized such that a pool of at least 15 individual faecal samples could be analysed. The developed RtF-RT-LAMP assay in our study could support the design and implementation of large-scaled epidemiological surveys as well as active surveillance and monitoring programs for effective control of PED.

References

Jan 1, 1978·Archives of Virology·M B Pensaert, P de Bouck
May 1, 1976·Journal of Medical Microbiology·G N WoodeG Jackson
Jan 1, 1995·Advances in Experimental Medicine and Biology·A UtigerM Ackermann
May 29, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·C A Muñoz-ZanziS K Hietala
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Nov 29, 2001·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·S Y KimB K Park
Jul 30, 2002·Molecular and Cellular Probes·K NagamineT Notomi
May 7, 2009·Epidemiology and Infection·M E Arnold, A J C Cook
Dec 2, 2009·The Journal of Molecular Diagnostics : JMD·Ralph StadhoudersMartin Schutten
Jan 21, 2012·Emerging Infectious Diseases·Rui-Qin SunChang-Xu Song
Oct 4, 2014·Emerging Infectious Diseases·Anastasia N VlasovaLinda J Saif
Nov 22, 2015·Preventive Veterinary Medicine·Yosuke SasakiAndres Perez

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Citations

Oct 5, 2020·Tropical Animal Health and Production·Thi Ngan MaiSatoshi Sekiguchi
Aug 28, 2020·Frontiers in Veterinary Science·Thi Ngan MaiSatoshi Sekiguchi
Aug 29, 2021·Viruses·Chao-Nan LinPeck Toung Ooi

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Datasets Mentioned

BETA
KT323979

Methods Mentioned

BETA
reverse transcription LAMP
electrophoresis
PCR
nucleic acid amplification

Software Mentioned

Primer Explorer
clustalW
StepOne

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