Development of Postmeiotic Cells In Vitro from Spermatogonial Cells of Prepubertal Cancer Patients

Stem Cells and Development
Maram Abofoul-AzabMahmoud Huleihel

Abstract

Aggressive chemotherapy in childhood often results in testicular damage and consequently jeopardizes future fertility. The presence of spermatogonial cells (SPGCs) in the testes of prepubertal cancer patient boys (PCPBs) can be used to develop future strategies for male fertility preservation. In the present study, we examined the presence of SPGCs in testes of chemotherapy-treated PCPBs and their ability to develop spermatogenesis in vitro using a three-dimensional culture system. Seven testicular biopsies were obtained from chemotherapy-treated PCPBs and one from a patient with β-thalassemia major. Isolated testicular cells were cultured in a methylcellulose culture system (MCS)-containing StemPro enriched with growth factors for 5-15 weeks. The presence of premeiotic, meiotic, and postmeiotic cells was examined by immunofluorescence staining and/or reverse transcription-polymerase chain reaction (RT-PCR) analysis. We observed SPGCs in the examined testicular biopsies. Isolated testicular cells cultured in MCS developed into colonies and contained premeiotic, meiotic, and postmeiotic cells. Furthermore, we identified sperm-like cells that had developed from testicular cells of a PCPB. Our results demonstrate, for the first ti...Continue Reading

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Citations

Sep 29, 2019·Andrology·A Gauthier-FisherC L Librach
Nov 7, 2019·International Journal of Molecular Sciences·Maxime VermeulenChristine Wyns
Jan 27, 2019·International Journal of Molecular Sciences·Maram Abofoul-AzabMahmoud Huleihel
Jun 13, 2020·Human Reproduction Open·E GoossensJ B Stukenborg
Dec 19, 2019·Journal of Clinical Medicine·Ylenia DucaAldo E Calogero
Feb 26, 2020·International Journal of Molecular Sciences·Marion DelessardNathalie Rives
Dec 7, 2018·International Journal of Molecular Sciences·Ali AbuMadighemMahmoud Huleihel
Nov 13, 2020·World Journal of Stem Cells·Han-Chao LiuGui-Hua Liu

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Methods Mentioned

BETA
biopsies
biopsy
PCR

Software Mentioned

MCS

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