Development of primers for detection of meat and bone meal in ruminant feed and identification of the animal of origin

Journal of Food Protection
T KusamaKoh-ichi Kadowaki

Abstract

The safe use of cattle feed free from meat and bone meal is an important prerequisite to prevent further spread of bovine spongiform encephalopathy. We designed primers to detect very small amounts of meat and bone meal in ruminant feed. Mitochondrial subunit 8 of the ATP synthase gene was used as a target sequence. PCR-based assays revealed amplification of DNA from mammals, ruminants, and individual species using these primers. The method allowed detection of the presence of meat and bone meal in ruminant feed from 0.1 to 0.01%. Sensitivity and effectiveness of the method for detecting prohibited animal proteins in ruminant feed was evaluated.

Citations

Sep 26, 2006·Journal of Food Protection·Jeong Chul HaTae Wha Moon
Aug 18, 2009·Journal of Food Protection·Naoki ShinodaKatsuaki Sugiura
Mar 9, 2011·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Naoki ShinodaKatsuaki Sugiura
Nov 30, 2006·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Tetsuya NomuraKoh-ichi Kadowaki
Apr 9, 2008·Journal of Food Protection·Irene MartínRosario Martín
Aug 9, 2011·Journal of Food Protection·Nicolette PegelsRosario Martín
Jun 13, 2009·Journal of Food Protection·Saira CawthrawScott D Reaney
Feb 26, 2010·Nucleosides, Nucleotides & Nucleic Acids·Naoki ShinodaKatsuaki Sugiura
Jan 7, 2005·Journal of Food Protection·Atsushi ToyodaHideo Yano
May 14, 2009·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Tomotaro YoshidaKatsuaki Sugiura

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