Development of radioactive tracing coupled with LC/MS-IT-TOF methodology for the discovery and identification of diaveridine metabolites in pigs.

Food Chemistry
Liye WangLing-Li Huang

Abstract

We developed a sensitive and reliable method by coupling radiotracing with LC/MS-IT-TOF to identify diaveridine metabolites. Tritium-labeled diaveridine was orally administered to pigs and their organs, blood, bile, and excreta were collected. Under optimized conditions, radioactive recovery was >90% and the highest numbers of metabolites were detected. MCX-based solid-phase extraction was conducted for urine, plasma, and bile purification. Methanol-chloroform 1:1 (v/v), methanol-chloroform 6:1 (v/v), methanol, methanol-chloroform 1:1 (v/v), and methanol were used as solvents to extract feces, liver, kidney, fat and muscle, respectively. The method validation confirmed satisfactory 3H-H exchange efficiency (<5%), chromatographic column efficiency (≥97.5%), LOQ (10.73 μg/kg), and analytical accuracy (97.6-107.8%) and precision (RSD < 5%). Moreover, novel in vivo metabolites were detected in the pigs, including D2 (3'-desmethyl-diaveridine monoglucuronide), D3 (diaveridine monoglucuronide). Hence, the analytical method developed herein lays an empirical foundation for further systematic studies of the diaveridine metabolism.

References

Jan 29, 2002·Environmental and Molecular Mutagenesis·Tomoko Ono-OgataHideo Yamagata
Dec 20, 2005·Biochemical Pharmacology·Stephen HawserKhalid Islam
Jun 23, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Christopher L ShafferGwendolyn D Fate
Dec 24, 2009·Antimicrobial Agents and Chemotherapy·Johannes ZanderThomas A Wichelhaus
Oct 27, 2010·Toxicology Letters·Zhao-Ying LiuZong-Hui Yuan
Sep 1, 1997·Environmental Toxicology and Pharmacology·T OnoT Ohta
Feb 22, 2012·Analytical and Bioanalytical Chemistry·Markus R Meyer, Hans H Maurer
Mar 1, 2012·Chemical Research in Toxicology·Emre M IsinLars Weidolf
Apr 15, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Juan LiZonghui Yuan
Jul 8, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hui WangXianhui Huang
Nov 19, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Thomas H HutchinsonColin H Walker
May 15, 2015·Journal of Agricultural and Food Chemistry·Huang LingliYuan Zonghui
Jul 26, 2015·Regulatory Toxicology and Pharmacology : RTP·Xu WangZonghui Yuan
Feb 4, 2016·Scientific Reports·Liye WangZonghui Yuan
May 17, 2019·Journal of Agricultural and Food Chemistry·Huiling TanLingli Huang
Jun 4, 2019·Parasitology Research·Sandra NoackPaul M Selzer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.