Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system

Cancer Research
Kensuke YamauchiRobert M Hoffman

Abstract

With the use of dual-color fluorescent cells and a highly sensitive whole-mouse imaging system with both macro-optics and micro-optics, we report here the development of subcellular real-time imaging of cancer cell trafficking in live mice. To observe cytoplasmic and nuclear dynamics in the living mouse, tumor cells were labeled in the nucleus with green fluorescent protein and with red fluorescent protein in the cytoplasm. Dual-color cancer cells were injected by a vascular route in an abdominal skin flap in nude mice. The mice were imaged with an Olympus OV100 whole-mouse imaging system with a sensitive CCD camera and five objective lenses, parcentered and parfocal, enabling imaging from macrocellular to subcellular. We observed the nuclear and cytoplasmic behavior of cancer cells in real time in blood vessels as they moved by various means or adhered to the vessel surface in the abdominal skin flap. During extravasation, real-time dual-color imaging showed that cytoplasmic processes of the cancer cells exited the vessels first, with nuclei following along the cytoplasmic projections. Both cytoplasm and nuclei underwent deformation during extravasation. Different cancer cell lines seemed to strongly vary in their ability to e...Continue Reading

References

Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·M YangR M Hoffman
Oct 11, 2001·Clinical & Experimental Metastasis·S PakuJ Timár
Aug 3, 2002·Nature Reviews. Cancer·Ann F ChambersIan C MacDonald
Nov 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Meng YangRobert M Hoffman
Mar 25, 2004·The American Journal of Pathology·Claire LugassyRaymond L Barnhill
Oct 1, 2005·Nature Reviews. Cancer·Robert M Hoffman

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Citations

Jan 12, 2008·Cancer Immunology, Immunotherapy : CII·S H Beachy, E A Repasky
Feb 25, 2011·International Journal of Clinical Oncology·Norio YamamotoRobert M Hoffman
Sep 13, 2008·Clinical & Experimental Metastasis·Robert M Hoffman
Jul 31, 2008·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Sharmeela KaushalMichael Bouvet
Nov 19, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Heiying JinYijiang Ding
Oct 31, 1991·Biochemical and Biophysical Research Communications·K HayasakaK Uyemura
Dec 22, 2009·Nature Medicine·Yvonne KienastFrank Winkler
Mar 29, 2011·Nature Medicine·Kristoffer WeberBoris Fehse
Apr 5, 2007·Nature Protocols·Robert M Hoffman, Meng Yang
Apr 5, 2007·Nature Protocols·Robert M Hoffman, Meng Yang
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hiroyuki KishimotoRobert M Hoffman
Jan 5, 2007·BMC Biotechnology·Adriaan D BinsTon N M Schumacher
Jun 10, 2010·Journal of Cell Science·Konstantin StoletovRichard Klemke
Jan 19, 2011·Journal of Cell Science·Evelyne BeerlingJacco van Rheenen
Nov 23, 2013·PloS One·Hiroyuki KishimotoRobert M Hoffman
Apr 18, 2014·Nature Reviews. Cancer·James R W ConwayPaul Timpson
Jun 29, 2014·The Journal of Surgical Research·Shinji MiwaRobert M Hoffman
Oct 30, 2009·Journal of Drug Targeting·Robert PolaBlanka Ríhová
Sep 1, 2010·Expert Opinion on Drug Discovery·Robert M Hoffman
Oct 31, 2015·Journal of Cellular Biochemistry·Yong ZhangNaohiko Seki
Apr 11, 2012·The Journal of Surgical Research·Masashi MomiyamaRobert M Hoffman
Jul 5, 2011·Journal of Neuroscience Methods·Sabine ScheibeJochen Herms
Dec 25, 2009·The Journal of Allergy and Clinical Immunology·Akihiro HasegawaToshinori Nakayama

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