Development of reconstituted embryos derived from somatic cell nuclei in the rabbit

Human Cell
Koji HosakaKahei Sato

Abstract

Production of cloned laboratory animals is helpful in the establishment of medical models. In this study, we examined to produce reconstituted embryos derived from somatic cell nuclei, and to establish embryonic stem (ES) cell lines from the embryo in rabbits. Metaphase II (M-II) oocytes from superovulated rabbit were used as nuclear recipients. Nuclear donor cells were fibroblasts collected from a Dutch Beleted rabbit. The M-II chromosome and the 1st polar body were aspirated, and a fibroblast was inserted into the perivitelline space of the enucleated oocyte. The pairs were electrofused for cell membrane fusion using a cell fusion apparatus, and reconstituted embryos were produced. The embryos were activated and cultured in modified HTF medium and DMEM. The embryos developed to the blastocyst stage were removed their zona pellucida, and they were cultured on the feeder cell layer. As a result of having observed development of reconstituted embryos, 21.2% of the embryos were developed to the blastocyst stage. In the embryos cultured on the feeder cells, the adhesion on feeder cells was observed. We obtained inner cell mass (ICM) colony derived from reconstituted embryos. At present, we are investigating to establish the ES cel...Continue Reading

References

Feb 27, 1997·Nature·I WilmutK H Campbell
Sep 19, 2000·Nature·I A PolejaevaK H Campbell
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lars M BjorklundOle Isacson

❮ Previous
Next ❯

Citations

Jul 23, 2005·Human Cell·Kahei SatoHisataka Hasegawa
Dec 10, 2009·Folia Histochemica Et Cytobiologica·Nurullah Keklikoglu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.