PMID: 9525781Apr 3, 1998Paper

Development of reference procedures for broth microdilution antifungal susceptibility testing of yeasts with standardized endpoint determination

Microbiology and Immunology
K MakimuraH Yamaguchi

Abstract

Standard guidelines for the broth microdilution antifungal susceptibility testing of amphotericin B, flucytosine, fluconazole, miconazole and itraconazole are reported. These are a modification of the method developed by the National Committee for Clinical Laboratory Standards (NCCLS) on the following two points: standardization of the means of endpoint determination and the inclusion of miconazole and itraconazole in the testing. MIC was determined to be when the positive control had a turbidity of 0.2 at the 630 nm wavelength. The endpoint was 80% inhibition for azoles and 100% inhibition for other drugs. The method provided good reproducibility, and a wide range of MIC distribution was observed in all antifungal agents except amphotericin B.

References

Dec 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J Wüst, U Hardegger
Aug 1, 1992·Antimicrobial Agents and Chemotherapy·R TellierI Campbell
Apr 1, 1995·Antimicrobial Agents and Chemotherapy·J H RexE J Anaissie
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Citations

May 10, 2001·Microbiology and Immunology·S InouyeH Yamaguchi
Jan 18, 2002·Antimicrobial Agents and Chemotherapy·Yasuki KamaiShogo Kuwahara
Oct 4, 2006·Biological & Pharmaceutical Bulletin·Jing-Xia ZhaoYuan-Ying Jiang
Sep 17, 1999·Journal of Clinical Microbiology·B A Arthington-SkaggsC J Morrison

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