Development of reporter gene imaging techniques for long-term assessment of human circulating angiogenic cells

Biomedical Materials
Yan ZhangErik J Suuronen

Abstract

The use of biomaterials and tracking the long-term fate of the transplanted cells is expected to help improve the clinical translation of cell therapies for cardiac regeneration. To this end, reporter gene strategies are promising for monitoring the fate of cells transplanted with or without a delivery biomaterial; however, their application with primary adult progenitor cells (such as human circulating angiogenic cells (CACs)) has not been extensively evaluated. In this study, human CACs were transduced with reporter genes via one of two lentiviral (LV) vectors: LV-GFP-iresTK or LV-Fluc-RFP-tTK. The mean transduction efficiency was 15% (LV-GFP-iresTK) and 13% (LV-Fluc-RFP-tTK) at multiplicities of infection (MOI) of 10 and 50, respectively. Western blot analysis confirmed HSV1-tk protein expression in transduced CACs. There was no significant difference in viability between the transduced CACs and the untreated controls at a MOI of 50 or below. However, a reduction was observed in cell viability of CACs transduced with LV-Fluc-RFP-tTK at an MOI of 100. Cell migration and angiogenic potential were not affected by transduction protocol. After 4 weeks, 80.3 ± 8.4% of the labeled cells continued to express the reporters and could ...Continue Reading

References

Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·S S GambhirR Blasberg
May 29, 2002·Tissue Engineering·Astrid HammAnja K Bosserhoff
Sep 28, 2002·Journal of Magnetic Resonance Imaging : JMRI·Vijay SharmaDavid Piwnica-Worms
May 3, 2003·Cancer Cell·Juri Gelovani Tjuvajev, Ronald G Blasberg
Jun 5, 2003·The Journal of Clinical Investigation·Ronald G Blasberg, Juri Gelovani Tjuvajev
Oct 29, 2003·Current Opinion in Chemical Biology·John V Frangioni
Dec 9, 2003·Nature Biotechnology·Sungjee KimJohn V Frangioni
Dec 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yu-An CaoChristopher H Contag
Feb 20, 2004·Cancer Research·Pritha RaySanjiv S Gambhir
Feb 7, 2006·Nuclear Medicine and Biology·Takako FurukawaYasuhisa Fujibayashi
Mar 10, 2006·Current Cardiology Reports·Ahmad Y Sheikh, Joseph C Wu
Apr 5, 2007·Nature Protocols·Gustavo TiscorniaInder M Verma
May 1, 2007·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Vladimir PonomarevJuri Gelovani
Jan 9, 2009·Current Pharmaceutical Design·Yan ZhangJean N DaSilva
Jul 21, 2009·Nature Protocols·Ning SunJoseph C Wu
Feb 20, 2010·The Journal of Thoracic and Cardiovascular Surgery·Yan ZhangMarc Ruel
May 17, 2011·Journal of Molecular and Cellular Cardiology·Drew KuraitisErik J Suuronen
Jun 3, 2011·Clinical Research in Cardiology : Official Journal of the German Cardiac Society·David M LeistnerBirgit Assmus
Oct 1, 2011·Circulation Research·Vincent F M Segers, Richard T Lee
Feb 22, 2012·Circulation Research·Gian Paolo FadiniStefanie Dimmeler
Apr 28, 2012·Theranostics·Gang Niu, Xiaoyuan Chen
Oct 12, 2012·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Jose S GilHarvey R Herschman
Jan 8, 2013·Nature Biotechnology·Hak Soo ChoiJohn V Frangioni
Aug 6, 2013·Mayo Clinic Proceedings·Milica Radisic, Karen L Christman
Apr 8, 2014·Cell Stem Cell·Patricia K NguyenJoseph C Wu
Jul 30, 2014·Methods in Molecular Biology·Aleksandra OstojicErik J Suuronen
Aug 6, 2014·Nature Biotechnology·Anna V NaumovaJoseph A Frank
Sep 23, 2014·Seminars in Nuclear Medicine·Tim Wollenweber, Frank M Bengel
Dec 4, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Brian McNeillErik J Suuronen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.